水稻花粉的离体萌发

In vitro Pollen Germination of Rice ( Oryza sativa L.)

选出适合于水稻花粉萌发的液体培养基 :2 0 %蔗糖 +10 % PEG4 0 0 0 +40 mg.L-1H3BO3+3mmol/ L Ca( NO3) 2 +10 mg.L-1VB1,得到 80 %的萌发频率。离体萌发时 ,花粉管生长很快 ,在 30 min内可达 70 0 μm。最适萌发的温度范围较宽 ,2 7～

A liquid medium good for rice pollen germination has been developed through a procedure of optimization. Over 80% of pollen germination rate was obtained with the medium composed of 20% sucrose, 10% PEG, 3m mol/L Ca (NO 3) 2, 10 mg VB 1 and 40 mg·L -1 boric acid. After a short time of hydration between 3～8 min, the pollen tube emerged and increased at the rate over 5.0 μm·min -1 in 30 min. The optimal incubation temperature range for the high germination was from 27 to 33℃.