摘要
选出适合于水稻花粉萌发的液体培养基 :2 0 %蔗糖 +10 % PEG4 0 0 0 +40 mg.L-1H3BO3+3mmol/ L Ca( NO3) 2 +10 mg.L-1VB1,得到 80 %的萌发频率。离体萌发时 ,花粉管生长很快 ,在 30 min内可达 70 0 μm。最适萌发的温度范围较宽 ,2 7~
A liquid medium good for rice pollen germination has been developed through a procedure of optimization. Over 80% of pollen germination rate was obtained with the medium composed of 20% sucrose, 10% PEG, 3m mol/L Ca (NO 3) 2, 10 mg VB 1 and 40 mg·L -1 boric acid. After a short time of hydration between 3~8 min, the pollen tube emerged and increased at the rate over 5.0 μm·min -1 in 30 min. The optimal incubation temperature range for the high germination was from 27 to 33℃.
出处
《作物学报》
CAS
CSCD
北大核心
2000年第5期609-612,共4页
Acta Agronomica Sinica
基金
国家自然科学基金!
EC:INCO- international cooperation with developing countries(1994~ 1998) funded projects
国家教委跨世纪
关键词
花粉萌发
水稻
花粉管
萌发频率
离体萌发
Rice
Pollen
in vitro pollen germination
Pollen tube
Germination rate.