基于Sinofiler检测系统的福建汉族群体遗传多态性研究

A study on genetic polymorphism of Han population in Fujian based on Sinofiler detection system

目的 调查福建地区汉族群体15个STR基因座遗传多态性参数,同时评估Sinofiler荧光标记复合扩增系统在福建地区汉族群体中的法医学应用价值.方法 应用Sinofiler荧光标记复合扩增系统检测500个福建地区汉族无关个体的15个STR 基因座的多态性,统计该群体各项遗传学参数.结果 15个STR基因座的基因型分布均符合Hardy-Weinberg平衡（HWE）定律.15个STR遗传标记均具有高度多态性,匹配概率为0.033～0.140,个体识别力为0.860～0.967,多态性信息含量为0.70～0.85,非父排除率（PPE）为0.492～0.774,纯合度为0.110～0.261,杂合度为0.739～0.890.发现18个稀有等位基因.结论 Sinofiler检测系统的15个STR基因座在福建地区汉族群体中具有较高的遗传多态性.

Objective To investigate genetic polymorphism parameters of 15 STR loci of Han population in Fujian region and evaluate the forensic application value of Sinofiler fluorescent multiplex amplification system in them. Methods Sinofiler fluorescent multiplex amplification system was used to detect 15 STR loci polymorphism of 500 Han unrelated individuals in Fujian region. Their genetic parameters were calculated. Results 15 STR loci genotype distributions were found to conform with the Hardy-Wein- berg equilibrium（HWE） laws. 15 STR genetic markers showed highly polymorphic,and their matching probabilities were 0. 033- 0. 140, discrimination powers were 0. 860-0. 967, polymorphism information contents were 0. 700-0. 850, probabilities of paternity exclusion（PPE） were 0. 492-0. 774,homozygosity were 0. 110-0. 261 and heterozygosities were 0. 739-0. 890. 18 rare alleles were found. Conclusion 15 STR loci in Sinofiler system shows highly genetic polymorphism in Han population in Fujian region.