乙型肝炎病毒DNA与“乙肝两对半”模式关系的临床研究

A clinical research of relationship of hepatitis B virus DNA and the“two and a half pairs of hepatitis B”

目的探讨乙型肝炎病毒(HBV)DNA与"乙肝两对半"模式的关系。方法选择240例乙型病毒性肝炎患者为研究对象,分别采用酶联免疫吸附测定(ELISA)及荧光定量聚合酶链反应(FQ-PCR)检测"乙肝两对半"及HBV-DNA,并对二者的相关性进行分析。结果不同"乙肝两对半"模式的患者HBV-DNA阳性率及含量不同,差异均有统计学意义(P<0.05)。HBsAg(阳性)、HBeAg(阳性)和HBcAb(阳性)模式的患者HBV-DNA阳性率及含量最高,分别为95.68%、(6.72±1.56)pg/mL。两两比较,HBeAg(阳性)模式的患者HBV-DNA阳性率及含量均高于HBeAg(阴性)模式(P<0.05)。"乙肝两对半"模式与HBV-DNA阳性率有明显相关性(χ2=36.78,r=0.30,P<0.05)。结论 "乙肝两对半"模式与HBV-DNA相互弥补,可作为乙型病毒性肝炎早期诊断、疗效评估的可靠指标。

Objective To investigate the relationship of hepatitis B virus（HBV） DNA and the ＂two and a half pairs of hepatitis B＂ models. Methods Two hundred and forty patients with hepatitis B were enrolled as study object. Enzyme-linked immunosorbent assay（ELISA） and fluorescent quantitative polymerase chain reaction（FQ-PCR） were employed to detect the ＂two and a half pairs of hepatitis B＂ and HBV-DNA, respectively, and their correlation were analyzed. Results Patients with different ＂two and a half pairs of hepatitis B＂ models showed different positive rates and contents of HBV-DNA with statistically significant difference（P〈 0.05）. The positive rate and content of HBV-DNA in patients with HBsAg（positive）, HBeAg（positive） and HBcAb（positive） models were the highest, which were 95.68 %, （6.72 ± 1.56）pg/mL, respectively. According to pair-wise comparison, the positive rates and contents of HBV-DNA in patients with HBeAg（positive） model were both higher than those with HBeAg （negative） model（P〈0.05）. The ＂two and a half pairs of hepatitis B＂ models markedly correlated with the positive rates of HBV-DNA（Х^2 = 36.78,r= 0. 30,P〉0. 05）. Conclusion The ＂two and a half pairs of hepatitis B＂ model and HBV-DNA may complement each other and act as reliable indicators of early diagnosis and curative effect evaluation of hepatitis B.