流感病毒亚型鉴定的两种方法比较

Comparison of two identification methods of influenza virus subtypes

目的比较直接免疫荧光法(DFA)与间接免疫荧光法(IFA)检测流感病毒亚型的特异性和敏感性,评价2种方法在流感病毒亚型检测中的应用效果。方法采用DFA法和IFA法对90份流行性感冒患者及120份健康者的鼻炎分泌物、血清进行检测。结果 DFA法检测腺病毒的阳性率为17%(36/210),IFA法检测的阳性率为26%(56/210),二者差异无统计学意义(P>0.05)。DFA法检测的敏感性为54.6%,特异性为97.2%;IFA法检测的敏感性为87.5%,特异性为48.2%。二者敏感性和特异性比较,差异有统计学意义(P<0.05)。结论 DFA与IFA法检测流感病毒具有较好的特异性和敏感性。

Objective To compare the specificity and sensitivity of direct immunofluorescence （DFA） and indirect immunofluo- rescence assay （IFA） in influenza virus subtypes detection,and their application effects. Methods DFA method and the IFA meth od were used to detect rhinitis secretions and serums of 90 patients with influenza and 120 healthy people. Results The positive rate of DFA detection for adenovirus was 17 % （36/210）, and that of IFA was 26 % （56/210）, with no statistically significant difference （P〉0.05）. The sensitivity and specificity of DFA were 54.6 % and 97.2 %, respectively. The sensitivity and specificity of IFA were 87.5 % and 48.2 %, respectively. Compared with their sensitivity and specificity, the differences were no statistical significance（P〈 0.05）. Conclusion DFA and IFA detection for influenza viruses have higher sensitivity and specificity.