唾液溶菌酶含量与大学生龋病关系研究

Relationship between concentration of lysozyme in saliva and dental caries in college students.

目的：探讨唾液溶菌酶含量与大学生患龋的关系，为大学生龋病预防提供依据。方法于2012年4月在中国医科大学在校大学生中随机选取65名（男32名，女33名），按龋失补牙数（DMFT）将研究对象分为无龋组（DMFT=0）22名、低龋组（DMFT=1~4）20名、高龋组（DMFT≥5）23名。用吐唾法收集受试者非刺激性全唾液3 mL作为样本，采用人溶菌酶ELISA试剂盒测定唾液溶菌酶含量，比较3组大学生唾液溶菌酶含量的差异。结果高龋组非刺激性全唾液采集成功率（65.2%）虽然低于无龋组（90.9%），但差异无统计学意义（P〉0.05）；非刺激性全唾液中溶菌酶含量无龋组[（10.15±2.60）μg/mL]显著高于高龋组[（8.52±2.03）μg/mL]，差异有统计学意义（P〈0.05）；3组大学生不同性别间唾液溶菌酶含量差异均无统计学意义（P〉0.05）。结论唾液溶菌酶含量与大学生龋病有关。

Objective To explore the relationship between concentration of lysozyme in saliva and dental caries in col-lege students in order to provide basis for its prevention. Methods Totally 65 healthy subjects from China Medical Uni-versity（33 female and 32 male）were randomly selected and participated in this study. Twenty-three high caries college students（DMFT ≥ 5）,twenty low caries college students（DMFT = 1-4）and twenty-two no caries college students （DMFT=0）were sampled. Salivary lysozyme was measured by ELISA Kit for the quantitative analysis of human lyso-zyme. Results The rate of success for collecting unstimulated whole saliva in high caries group（65.2%）was lower than that in no caries group（90.9%）,but there was no statistical difference（P〈0.05）;The concentration of lysozyme in no caries group[（10.15 ± 2.60）μg/mL]was higher than that in high caries group[（8.52 ± 2.03）μg/mL]（P〈0.05）. How-ever,no significant gender difference was found in no caries group and low caries group and high caries group（P〉0.05）. Conclusion Saliva lysozmye is related to college students＇ caries.