氧化应激与牙周病易感性的相关分析

Oxidative stress and susceptibility of periodontal disease

目的:通过膳食营养素摄入调查及血液、唾液样本抗氧化指标检测,探讨饮食抗氧化营养素摄入和机体抗氧化水平与牙周病发生、发展及氧化应激与牙周病的关系。方法:随机选择50例中重度牙周病患者及50例牙周组织正常者进行膳食频率和72 h膳食回顾调查,并收集其血液和唾液样本,采用液相色谱与质谱联用技术检测其中营养素及抗氧化相关指标,采用SPSS19.0软件包对数据进行配对t检验。结果:膳食频率调查显示,牙周病组的薯类、水(海)产品、鲜奶、大豆类、新鲜蔬菜、新鲜水果及维生素类膳食补充剂的摄入量显著低于正常对照组(P<0.05);72 h膳食回顾调查显示,牙周病组的维生素A、B2、C、E,钙、镁、铜、硒的摄入量均显著低于正常对照组(P<0.05);样本检测结果显示,牙周病组血液及唾液超氧化物歧化酶水平显著低于正常对照组(P<0.05);牙周病组唾液镁、钙、锰及锌水平显著低于正常对照组(P<0.05)。结论:牙周病的发生、发展与氧化应激相关,膳食抗氧化营养素对牙周组织具有保护作用。

PURPOSE： To investigate the relationship between dietary antioxidant status and susceptibility of periodontal disease in humans. METHODS： Fifty patients with moderate-to-sever periodontal disease and 50 subjects without periodontal disease were enrolled in this study. Food frequency questionnaire and 72-hour diet recall were conducted to study the dietary pattern and antioxidant nutrients intake in patients and healthy subjects. Nutritional status including vitamins ,mineral substance, and antioxidant enzymes in these 2 groups were analyzed in serum and saliva samples by liquid chromatography-mass spectrometry （LC-MS）. SPSS 19.0 software package was used for paired t test. RESULTS： Compared with the control group, the chronic periodontal group had lower levels of potatoes, aquatic product, milk, soy, fresh vegetables, fruit and vitamin supplements intake （P〈0.05）. Through food components analysis we found that the intake of vitamin A, C, B2, E, calcium, magnesium, copper, selenium was significantly lower in the chronic periodontal disease group than that in the control group （P〈0.05）. Biochemical analysis demonstrated that antioxidant nutrient levels including antioxidant enzyme SOD in serum and saliva together with magnesium, calcium, manganese, zinc in saliva were significantly lower in disease periodontal group than in the control group （P〈0.05）. CONCLUSIONS： Oxidative stress plays an essential role in the occurrence and development of periodontal disease. The antioxidant nutrients may help to protect paradentium. Supported by Research Fund from Shanghai Municipal Health Bureau （20114103）.