紫杉醇在体外对二磷酸腺苷诱导的血小板聚集的影响

Effect of Paclitaxel on Platelet Aggregation Induced by Adenosine Diphosphate in vitro

目的研究紫杉醇在体外对二磷酸腺苷(ADP)诱导的血小板聚集的影响。方法血小板混悬液中加入不同浓度〔0(对照组)、0.01ng/mL、0.1ng/mL、1ng/mL、10ng/mL、100ng/mL、1 000ng/mL〕的紫杉醇,在最佳ADP终浓度和最佳孵育时间条件下,分别测定血小板聚集率。结果在ADP终浓度为10μmol/L、孵育时间为10min条件下,随着紫杉醇浓度的增高,其促进ADP诱导的血小板聚集的作用也随之增加,不同浓度紫杉醇间血小板聚集率的比较差异具有统计学意义(P<0.05)。结论紫杉醇能在体外增强ADP诱导的血小板聚集,且诱导作用呈浓度依赖性。

Objective To investigate the effect of paclitaxel on platelet aggregation induced by adenosine diphosphate （ADP） in vitro. Methods Platelet suspension was incubated with paclitaxel in different concentrations （0 ng/mL, 0.01 ng/mL, 0. 1 ng/mL, 1 ng/mL, 10 ng/mL, 100 ng/mL, 1 000 ng/mL）, then the maximal extent of platelet aggregation was determined under the conditions of the best ADP concentration and the best incubation time. Results Under the condition of 10 μmol/L ADP and incubated for 10 rain, with the increase of paclitaxel concentration, the effect of paclitaxel on platelet aggregation induced by ADP elevated accordingly, and the differences among the different concentration groups were statistically significant （P〈0.05）. Conclusion Paclitaxel can enhance ADP-induced platelet aggregation in a concentration-dependent manner in vitro.