微米六合丹外敷对急性胰腺炎大鼠氧化应激及炎症反应水平的影响

Effects of Micron Chinese Herbal Liu-He-Dan Ointment on Inflammation and Oxidative Stress in Rats with Acute Pancreatitis

目的探讨微米六合丹外敷对急性胰腺炎大鼠氧化应激及炎症反应水平的影响。方法将18只大鼠随机分为正常组、急性胰腺炎模型组、微米六合丹外敷组3组,每组6只。使用150g/L精氨酸腹腔注射造模,正常组注射等容无菌生理盐水。微米六合丹外敷组大鼠造模后腹部外敷微米六合丹72h后处死。留取血清及胰腺组织标本,测定血清淀粉酶、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6和IL-10水平、大鼠胰腺超氧化物歧化酶(SOD)及丙二醛(MDA)含量、胰腺组织病理学评分。结果与模型组比较,微米六合丹外敷能降低急性胰腺炎大鼠的血清淀粉酶、TNF-α、IL-6和IL-10水平,胰腺组织SOD含量升高(P均<0.05),MDA含量有降低趋势(P>0.05)。而胰腺组织病理评分差异没有统计学意义(P>0.05)。结论微米六合丹外敷有助于减轻实验大鼠急性胰腺炎过氧化损伤和炎症反应。

Objective To evaluate the effects of micron Chinese herbal Liu-He-Dan ointment （MLHD） on inflammation and oxidative stress in rats with acute pancreatitis （AP）. Methods Eighteen SD rats were randomized into three groups., normal group （n=6）, AP group （n=6） and MLHD group （n=6）. The rat model of AP was induced by intra-peritoneal injection of 150 g/L L-arginine with dosage of 2. 0 mg/g twice within 2 hours. As control, the normal rats were injected the same amount of saline. The MLHD was applied to the abdomen area of the AP rats in MLHD group for 72 h. The serum and pancreas of the rats were obtained. The level of amylase, TNF-α, IL-6, IL-10 in serum and SOD, MDA in the pancreas were detected and quantified. Results Compared with AP group, the serum levels of amylase, TNF-α, IL-6, IL-10 were significantly decreased in MLHD group （P 〈0.05）. And the SOD in pancreas increased significantly （P〈0. 05）, but a decreasing trend of MDA level in MLHD group （P〉0.05） was observed. The serum level of the pathological score of pancreas in treatment group were similar to that in the model group （P〉0.05）. Conclusion Micron Liu-He-Dan ointment may have therapeutic and protective effects on AP by antioxidation and alleviating inflammatory response.