TNF-α对损伤的内侧副韧带和前交叉韧带中BMP-1基因表达的影响

Effects of BMP-- 1 gene expression of TNF-- on injury of the medial collateral ligament and anterior cruciate ligament.

目的：就TNF—α对损伤的内侧副韧带和前交叉韧带中BMP-1基因表达的影响进行探讨。方法：选择重庆医科大学附属第一医院骨科教研室在2011年09-11月时间内所收集到的4例滑膜组织和交叉韧带标本，其中女2例，男2例，截肢膝关节相应组织是在无菌条件下用镊子和剪刀来取得。先细胞培养，其次，pET32a（＋）-Tat—PTD—VP3重组质粒构建示，分别测定半定量PCR、实时定量PCR。结果：本组资料研究表明，TNF-α作用时间分别为24h、12h、6h、2h，作用浓度为5ng／ml。对于那些已经损伤的MCL细胞中，BMP-1的表迭随着TNF-α浓度的不断增加也会出现避步的下降趋势，用实时定量PCR来进行显示，BMP-1表这为117％、70％、62％、41％。而对于那些已经损伤的ACL细胞中，BMP-1的表达随着TNF-α浓度的不断增加会出现逐渐下降的趋势。用实时定量PCR来进行显示，BMP-1表达从最初的69％，下降到最后的27％。结论：TNF-α能够有效地下调ACL作用，以及前交叉韧带（ACL）和内侧刮韧带（MCL）中BMP～1基因表述都出现了较大的下降，本实验为有效促进ACL自我修复，早期抑制TNF-α浓度提供了较好的理论依据。

Objective：To explore effects of BMP-1 TNF- on the injury of the medial collateral ligament and anterior cruciate ligament in gene expres- sion. Methods：4 cases of synovial tissue Department of orthopedics,First Affiliated Hospital of Chongqing Medical University collected in 2011：09 - 11 cruci- ate ligament specimens,including 2 cases of female,male 2 cases, amputation of knee joint corresponding tissue is achieved with tweezers and scissors under sterile conditions. Second, cell culture, pET32a （ ＋ ） to construct the recombinant plasmid of - Tat- PTD- VP3, semi- quantitative PCR, real- time quanti- tative PCR were measured. Results：the study shows that, TNF-α action time were 24h, 12h, 6h, 2h, concentration is 5ng＇ ml. For those who have damage in MCL cells,the expression of BMP-1 with increasing TNF- concentration will gradually decline, real-time quantitative PCR was used to display,BMP-1 expression was 117 %, 70 %,62 % ,41%. And to all those who have injured ACL ceils, the expression of BMP-1 with increasing TNF- concentration will gradually decline. Real--time quantitative PCR was used to show the expression of BMP-1, from the initial 69 %, down to the last 27 %. Conelusion： TNF-α can downregulate the ACL, and anterior eruciate ligament （ACL） and medial collateral ligament （MCL） expression of BMP-1 genes have appeared in a lar- ger drop,this experiment to effectively promote the ACL repair itself,the early inhibition of TNF-α concentrations provided a good theoretical has