TECA生物型人工肝支持系统替代衰竭肝脏功能机制的实验研究

EXPERIMENTAL STUDY OF TECA BIOARTIFICIAL LIVER SUPPORT SYSTEM IN TREATMENT OF ACUTE LIVER FAILURE CANINES

分析中国实验用小型猪肝细胞型生物人工肝支持系统 (BALSS)替代衰竭肝功能的可能机制。使少肝急性肝衰 (ALF)犬血液或 16 40培养液循环流经BALSS中空纤维管的内腔 ,与培养于中空纤维管外腔中的猪肝细胞营养液进行物质交换。测定 16 40液中利多卡因浓度、白蛋白和尿素浓度的变化 ;用蛋白电泳法测定 16 40液和猪肝细胞营养液中蛋白成分的变化 ;用透射电镜观察治疗ALF犬时BALSS中猪肝细胞超微结构的变化。结果显示BALSS循环 6h时 ,16 40液中利多卡因浓度明显降低 ;白蛋白和尿素浓度明显升高 ;分子量小于 10 0kD的蛋白质成分明显增多。BALSS治疗ALF犬 6h时 ,猪肝细胞的超微结构出现明显的损伤。提示此型BALSS的猪肝细胞通过透析膜可较好地发挥肝细胞的生物转化及合成代谢等功能 ,以此达到暂时替代衰竭肝脏功能的效应。

Abstract To analyse the mechanism of TECA bio-artificial liver support system(BALSS)to treat acute liver failure (ALF) canines. The blood of partial liver resection-induced ALF canines or RPMI1640 solution were circulated in the inner space of the hollow fibers (blood loop), and porcine hepatocytes were cultured in outer space of the hollow fibers (cell loop). The concentrations of lidocaine, albumine and urine in the RPMI1640 solution were measured. The compositions of proteins in cell loop and blood loop were analysed by polyacrylamide gel electrophoresis (PAGE). The ultrastructure of porcine hepatocytes in BALSS was observed with transmission electronic microscopy. There were not any proteins in RPMI1640 solution before BALSS circulation. After 6 hours of the BALSS circulation, the concentration of lidocaine in RPMI1640 solution decreased significantly,and concentration of albumine and urine nitrogen increased gradually. The ultrastructure of the porcine hepatocyte shown damaged after BALSS 6h. The results suggested that TECA BALSS could replace temporarily the functions of failure liver by the effects of biologic transform and synthesis of porcine hepatocytes.