TGFα、EGFR反义寡聚核苷酸对大肠癌生长的抑制作用

GROWTH INHIBITION OF COLORECTAL CANCER BY TGF-α ANTISENSE OLIGODEOXYNUCLEOTIDE AND EGFR ANTISENSE OLIGODEOXYNUCLEOTIDE

研究转化生长因子α(TGFα)、表皮生长因子受体 (EGFR)反义寡聚核苷酸 (ASODN)对人大肠癌生长增殖的作用。反义TGFα、反义EGFR及无关对照ODN经阳离子脂质体包裹后转染人大肠癌HR8348细胞。采用噻唑蓝比色法 (MTT法 ) ,细胞周期分析 ,裸鼠体内接种等方法测定瘤细胞体内外增殖的变化。与对照组HR8348细胞相比 ,经TGFα反义ODN处理的HR8348细胞体外增殖速度减慢 (抑制率达 80 % )、DNA合成受抑、裸鼠体内成瘤率下降 (2 5 %∶ 10 0 % )。经EGFR反义ODN处理的HR8348细胞也得到了类似的结果。TGFα反义ODN、EGFR反义ODN均能有效抑制大肠癌细胞的体内外生长增殖。

To investigate the effects of TGFαantisense oligodexynucleotides(ASODN) and EGFR ASODN on human rectal carcinoma cell line HR8348. Cationic liposome, lipofectamine, were selected as delivery vector HR8348 cells were treated with ASODN in vitro. HR8348 cells with or without pretreatment were inoculated into nude mice. The proliferation and DNA synthesis of HR8348 cells treated with TGF-α ASODN and EGFR ASODN were greatly inhibited, and tumorigenic rate was also greatly reduced. TGF-α ASODN and EGFR ASODN could inhibit the proliferation of human colon cancer cell line in vitro as well as in vivo, the results implicate. The potential value in colorectal cancer gene therapy.