活血消痛颗粒的质量标准研究

Study on the Quality Standard of Huoxuexiaotong Granule

目的：建立活血消痛颗粒的质量标准。方法：采用薄层色谱法对活血消痛颗粒中当归、苏木进行定性鉴别，并采用高效液相色谱法对方中丹参酮Ⅱ^进行含量测定研究。色谱柱：AgilentElipse-C18柱（4．6mml50mm，5μm）；流动相：甲醇-水（75：25）；流速：1．0ml／mia;柱温：35℃；检测波长：270nm。结果：薄层色谱斑点清晰，阴性无干扰；丹参酮ⅡA在浓度为2．-40μg／ml范围内呈良好的线性关系，r=0．9998，平均加样回收率为97．84％，RSD=1．98％（n=6）。结论：该方法简便、准确、可靠，可以作为活血消痛颗粒的质量控制方法。。

Objective： To establish quality standards for Huoxuexiaotong granule. Methods; TLC was used to distinguish Danggui, Sumu, HPLC was used for the determination of tanshinone II ^. The column was Agilent Elipse-C18 column（4.6mm150mm, 5μm） ; mobile phase were methanol - water （75： 25） ; the detection wavelength was at 270nm; flow rate at 1.0ml？ rain-1 and column temperature at 35℃. Results： The TLC spots were clear and negative sample is non--interference; tanshinone II^. show a good linear relationship in the range of 2.48-12.40μg/ml, r = 0. 9998, the average recovery was 97. 84M with RSD=1. 98% （n = 6）. Conclusion： The method is simple, accurate, reliable and can be used as quality control for Huoxuexiaotong granule.