摘要
对醋化醋杆菌产乙醛脱氢酶的发酵条件进行优化，得出最优培养条件如下：温度32℃、pH5．0、接种量10％、装液量50mL，此时，酶活为0．893U／mL。对其粗酶液的酶学特性的研究表明：该酶最适作用pH为6．0；pH5．0-6．0时酶的稳定性较好；最适作用温度为50℃；在25。45℃热稳定性较好；Ca2＋、Al3＋是酶的激活剂，K＋、Na＋、Mg2＋、Fe2＋、Ba2＋对酶有一定的抑制作用，Mn2＋、Zn2＋是该酶的强抑制剂。
The cultivation conditions of Acetobacter aceti were optimized to increase the ALDH activity. The optimal fermentation conditions were as follows： incubation temperature 32 ℃, pH value 5.0, inoculum 10%, liquid volume 50 mL. Under these conditions, the ADLH activity was 0.893 U/mL. Then the enzymatic properties of the crude enzyme were detailed studied. The results showed that： the optimal pH value of ALDH was 6.0; ALDH was stable in the pH range of 5.0-6.0; optimum temperature of ALDH was 50 ℃; thermal stability was good between 25 ℃ to 45 %. Ca2＋ and Al3＋ were the activator of ALDH, the enzyme was inhibited by K＋, Na＋, Mg2＋, Fe2＋ and Ba2＋, the enzyme was strongly inhibited by Mn2＋ and Zn2＋.
出处
《食品科技》
CAS
北大核心
2013年第11期24-28,共5页
Food Science and Technology
基金
国家自然科学基金项目(81273004)
徐州工程学院科研基金青年课题(XKY2012056)
徐州工程学院科研基金培育课题(XKY2010112)
关键词
醋化醋杆菌
乙醛脱氢酶
条件优化
酶学性质
Acetobacter aceti
Aldehye dehydrogenase
conditions optimization
enzymatic properties
