响应面法优化苦丁茶抑菌活性成分提取工艺

Optimization of the antimicrobial activity of Guangxi leaf of Chinese holly by using respond surface method

利用响应面分析法,以苦丁茶水提物抑菌活性为指标,对苦丁茶水提物的提取工艺进行了优化。在单因素试验基础上,选择提取温度、提取时间和液料比3因素为自变量,以杯碟法测定苦丁茶水提物对大肠杆菌的抑菌圈直径为响应值,根据中心组合(Box-Behnken)试验设计原理,采用3因素3水平的响应面分析法优化苦丁茶抑菌活性成分提取工艺研究。结果表明,苦丁茶水提物中抑菌活性成分提取的最佳工艺条件为:回流提取3次、提取温度98℃、提取时间2.15 h、料液比1:60。在此条件下提取液对大肠杆菌的抑菌圈直径达20.62 mm。

The extraction process of the aqueous extract with the antimicrobial activity from Ilex kudingcha C. J. Tseng was optimized by response surface methodology （RSM）. On the basis of the single-factor tests, extraction temperature, extraction time and solid/liquid ratio were selected as independent variables, and the inhibition zone diameter of the leaf of Chinese holly extract on E. coli was determined in response to the value with the cylinder plate method. The relationship between the antimicrobial ability and the above three factors was modeled using a 3-factor, 3-level Box-Behnken experimental design and further, the established model was analyzed by response surface methodology to obtain the optimum extraction conditions. The results showed that the optimum conditions for the the antimicrobial ability ofleaf of Chinese holly extraction were as follows： tree times of reflux extraction, the extraction temperature of 98 ~, the extraction time of 2.15 h and the solid/liquid ratio of 1：60 respectively. Under these conditions, the observed values of inhibition zone diameter on E. coli. was 20.62 mm.