记忆系统免疫毒性评价模型的建立

Establishment of Immunotoxicity Evaluation Model for Memory System

基于T细胞依赖性抗体实验思路，建立具有剂量关系的记忆系统免疫毒性评价模型。雌性SD大鼠分别在d1和d22尾静脉注射抗原钥孔戚血蓝素（KLH）300μg／kg，d22～d24灌胃给予环磷酰胺（1，5或20mg·kg-1d-1，连续3d）。结果表明，d27时1高剂量组大鼠外周血中白细胞、淋巴细胞和中性粒细胞数目比阴性组显著降低，而d30和d33时中性粒细胞数目增加。免疫酶联技术检测结果表明，1高剂量组大鼠特异性抗体（IgG）含量显著降低（P〈0．01），且具有时间、剂量依赖性。

Based on the T-cell dependent antibody response method, the immunotoxicity evaluation model for memory system with a dose-dependent relationship was established. The keyhole limpet hemocyanin （KLH） was injected to female SD rats at the dosage of 300 μg/kg at dl and d22, and these rats were ig administrated with cyclophosphamide （1） at the dosage of 5 or 20 mg.kg 1.d-1 for three days （from d22 to d24）. The results showed that the white blood cells, lymphocytes and neutrophils of the high-dose group of 1 were significantly decreased at d27 compared with the blank group, while the neutrophils were increased at d30 and d33. The results of ELISA showed that the serum IgG level of high-dose group of 1 were also significantly decreased （P〈0.01） in a time- and dose-dependent manner.