软膏制剂中挥发性活性成分的胶束-顶空气相色谱法测定

Determination of Volatile and Active Ingredients in the Ointment by Micellar Headspace Gas Chromatography

建立了胶束．顶空气相色谱法快速检测软膏制剂中的挥发性成分樟脑和薄荷脑。以0．1％Tween-80水溶液为顶空溶剂，在85℃的顶空温度下平衡20min以获得待测组分。采用DM．WAX毛细管柱，柱温125℃，进样口温度150℃，氢火焰离子化检测器温度180℃。樟脑和薄荷脑均在1～32μg／ml浓度范围内线性关系良好，回收率为101．3％～102．1％和97．0％～100．5％，RSD分别小于2．9％和314％。

A micellar headspace gas chromatography was established for the determination of camphor and menthol in the ointment. The analytes were equilibrated at oven temperation of 85 ℃ for 20 rain with the 0.1% Tween-80 as matrix medium. A separation was performed on a DM-WAX capillary column. The temperatures of column oven, injector and FID were 125,150 and 180 ℃, respectively. Their calibration curves were linear in the range of 1 - 32μg/ml. The recoveries of camphor and menthol were 101.3 % - 102.1% and 97.0% - 100.5%, with RSDs less than 2.9% and 3.4 %, respectively.