摘要
目的建立阿霉素聚乳酸微球中药物的含量测定方法。方法采用紫外分光光度法测定微球中阿霉素的含量。结果阿霉素溶液在7．5～15．0μg·ml^-1范围内线性良好，标准曲线回归方程为C=0．04007A＋0．00867，相关系数r=0．9997（n=6），阿霉素微球的平均载药量为5．93％，包封率为38．3％。结论该方法简便快速，灵敏度高，重复性好，结果准确，可用于测定阿霉素微球的含量。
Objective To establish a method for the determination of adriamycin content adriamycin poly lactic acid microspheres. Methods The ultraviolet spectrophotomethy （UV）method was used to analyze the content of adriamycin. Results The linearity of adriamycin was better in the rang of 7.5 to 15.0μg·ml^-1 ,C = 0.04007A ＋0.00867, r =0.9997 （ n = 6）. The loading amount was 5.93% and entrapment rate was 38. 3%. Conclusion This methed is simple, sensitive, accurate and repeatable, it can be used for the determination of adriamycin poly lactic acid microspheres.
出处
《湖北科技学院学报（医学版）》
2013年第5期374-376,共3页
Journal of Hubei University of Science and Technology(Medical Sciences)
基金
湖北科技学院校级科研项目(KY10061)
关键词
阿霉素
微球
紫外分光光度法
含量测定
Adriamycin
Microspheres
Ultraviolet spectrophotomethy method
Content determination
