胰腺癌K-ras基因12密码子突变方式的研究

A study on the mutation style of K-ras condon 12 in pancreatic adenocarcinoma

目的 阐明中国胰腺癌患者K -ras基因 12密码子突变方式。方法 采用 3′碱基特异性引物PCR技术分析 30例已证明存在突变的胰腺癌DNA标本。结果 检测到 4种突变方式即GAT、GTT、CGT和GCT ,比例分别为 5 2 .9%、32 .4%、8.8%和 5 .9%。结论 中国胰腺癌K -ras基因 12密码子存在 4种突变方式 。

Objective To investigate the mutation style of K-ras codon 12 in Chinese patients with pancreatic adenocarcinoma. Methods 30 DNA specimens of pancreatic adenocarcinoma which had been proved to have the mutation were analysed by 3′base special prime PCR method. Results Four styles were detected,i.e. GAT,GTT,CGT and GCT,of which the ratios were 52.9%,32.4%,8.8% and 5.9% respectively. Conclusion There are 4 styles of mutation of K-ras codon 12 in pancreatic adenocarcinomas in China, which might be varied with different geographical zones.