多枝柽柳乙酸乙酯提取物对小鼠皮肤伤口愈合的影响

Influence of Extract from T. Ramosissima on the Skin-wounded Mice

[目的]研究多枝柽柳乙酸乙酯提取物对小鼠创伤皮肤的影响。[方法]建立小鼠全层皮肤切除伤口模型,对小鼠进行不同浓度的多枝柽柳提取物灌胃,通过小鼠伤口愈合的情况、大体观察及组织切片HE染色观察多枝柽柳对小鼠皮肤伤口愈合影响。[结果]多枝柽柳乙酸乙酯提取物对皮肤创伤小鼠进行灌胃给药,创伤11d后测定未愈合伤口面积,高剂量组(17.68mm2),中剂量组(17.90mm2),低剂量组(20.18mm2)和对照组(31.48mm2)比较,均有显著差异。HE染色切片结果发现,实验组伤口完全愈合,上皮完整,有正常角质层形成,血管少,表皮下大量胶原形成,基本与表皮平行生长。[结论]多枝柽柳乙酸乙酯提取物对小鼠皮肤伤口愈合有一定的促进作用。

[Objective]To research the effect of acetic ether extract from T. ramosissima on the skin-wounded mice. [Methods]The acetic ether extract of T. ramosissima was administrated to skin-wounded mice by gavage methods. [Results] The experimental groups in different dosages had significant difference ,compared to the control group, with the areas of wound healing 17.68 mm^2, 17.90 mm^2, 20.18 mm^2 and 31.48 mm^2,after the acetic ether extract of T. ramosissima administration to skin-wounded mice by gavage methods. [Conclusions] It was suggested that the acetic ether extract of T. ramosissima had some protective effects on improving the wound healing.