阿苯达唑脂质体对泡状棘球蚴作用的病理形态学观察

Pathohistological changes on efficacy of liposomal albendazole against echinococcus multilocularis infection in experimental mice

目的 :试图通过阿苯达唑脂质体口服新剂型与阿苯达唑片剂的动物对比实验 ,对口服和腹腔注射抗泡状棘球蚴作用做出客观评价。 方法 :采用泡状棘球蚴继发感染小鼠动物模型 (2 2 5只 ) ,经灌胃和腹腔注射 (每公斤体重 37.5、75 .0和 15 0 .0 m g) 10周 ,观察其病理组织学变化。将用药后病理形态变化分为正常、变性和坏死 3级 ,以便用药组与对照组进行等级资料的统计学比较。结果 :阿苯达唑及代谢产物有较强的破坏棘球蚴囊壁结构和抑制原头蚴增殖作用。 结论 :阿苯达唑脂质体经口灌注或腹腔注射抗泡状棘球蚴作用均优于口服阿苯达唑片剂。

Objective: According to comparative animal experiments between the groups for new formulation(liposomal albendazole)and the albendazole tablets, anti echinococcus multilocularis effect has been evaluated objectively between oral path and intraperitoneal path. Methods: 225 NIH mice with echinococcus multilocularis infection were given albendazole orally and intraperitoneally (37.5 mg、37 mg and 150 mg /kg) for 10 weeks. In order to make statistical analysis, histopathological changes were divited into three levels such as normal、degeneration and necrosis. Result: albendazole and its metabolic product had effect in damaging the echinococcus multilocularis cysts and inhibiting the E.M protoscoleces multiplication. Conculusion: Groups treated by both oral irrigation path and intraperitoneal injection had more effect than that by oral tablets administration alone.