涎腺肿瘤中C-erbB-2、PCNA和p16蛋白的表达及临床病理学意义

The altered expression of C erbB 2\, PCNA and p16 in salivary tumors and its clinicopathologic significance

目的 :探讨抑癌基因 p16、原癌基因 C- erb B- 2、增殖细胞核抗原 (PCNA)在涎腺肿瘤中的异常表达与肿瘤病理生物学特征间的关系及临床意义 ,为涎腺恶性肿瘤有效防治提供实验科学依据。方法 :应用免疫组化 L SAB法检测涎腺恶性肿瘤 30例、涎腺多形性腺瘤 31例及涎腺非肿瘤组织 14例的 C- erb B- 2、PCNA及 p16蛋白的表达及变化。结果 :多形性腺瘤中 C- erb B- 2、PCNA、p16阳性率分别为 38.7%、71.0 %、77.4% ,均低于涎腺恶性肿瘤阳性率 76 .7%、86 .7%、80 .0 % ,但仅 C- erb B- 2两者间 P<0 .0 5。涎腺恶性肿瘤中 PCNA与 C- erb B- 2、p16间无相关性 (P >0 .0 5 )。结论 :(1)免疫组化法检测抑癌基因 p16、原癌基因 C- erb B- 2、PCNA在细胞内的表达可对良恶性病变从分子水平进行评估 ,并可作为早期诊断及临床治疗涎腺恶性肿瘤的分子生物学指标。 (2 ) C- erb B-

Objective: To study the altered expression of C erbB 2\, PCNA and p16 in human salivary tumors related to the biopathology and clinical sigificance. Methods: Express of C erbB 2\, PCNA and p16 in salivary malignant tumors (30), pleomorphic adenomas (31) and non tumor tissues (14) was detected by immunohistochemistry (LSAB). Results: The positive frequency of C erbB 2, PCNA and p16 in salivary maligant tumor was 76.7%, 86.7% and 80.0% revealing more higher than in pleomorphic adenomas (38.7%, 71.0% and 77.4%) and non tumor tissues (50.0%, 64.3% and 85.7%) except p16, respectively. However, only C erbB 2 expression showed a statistic signifisance (P<0.05). Among them no association of expression of C erbB 2, PCNA and p16 either in malignant or benign lesions was found. Conclusions: (1) Detection of C erbB 2, PCNA and p16 alteration may offer some molecular index for the early diagnosis, clinical therapy of salivary malignant and benign tumors. (2) C erbB 2 overexpression may involve a maligant transformation of benign lesions.