冻干与辐照前后人脐静脉同种异体抗原和组织结构的变化

The effects of freeze drying and irradiation on the allo antigen and tissue structure of human umbilical veins

目的 :寻找理想的同种异体小口径血管移植材料及其制备方法。 方法 :采用冻干与辐照方法处理人脐静脉血管 (HU V) ,通过光镜、电镜和免疫组织化学染色 ,观察冻干、辐照和二者联合处理对血管同种异体抗原和组织结构的影响。结果 :经单纯冻干处理可使人脐静脉主要组织相容性抗原 (MHC) I类抗原表达量减少 70 % (P <0 .0 1) ,冻干处理后 ,血管内膜完整 ,脱水使血管壁细胞外层基质呈网状支架样结构 ,但其胶原和弹力纤维都完好保存。单纯辐照处理可使人脐静脉 MHC I类抗原表达量减少 30 % (P <0 .0 5 ) ,经辐照后血管壁组织结构无明显改变。冻干 +辐照处理可使人脐静脉 MHC I类抗原表达量减少 85 % (P <0 .0 1) ,经冻干 +辐照处理后 ,内膜脱落 ,管壁支架结构基本保存完整 ,平滑肌细胞 (SMC)失去活力 ,胞浆结构消失 ,只残留肌膜和浓缩的胞核。 结论 :冻干 +辐照处理的人脐静脉抗原性明显降低 ,管壁结构保存基本完好 ,且具有来源丰富、无分枝、无瓣膜等优点 ,是很有发展前途的生物性血管移植材料 ,值得临床进一步推广应用。

s\ Objective: To search for an ideal allograft as small artery bypass graft. Methods: To modify the human umbilical vein (HUV) allografts by the methods of freeze drying and irradiation, and investigate the changes of its structure and allo antigen expression. Results: The freeze drying and irradiation could reduce the expression of MHC I 70% and 30 % respectively. Combination with both of the methods could reduce the expression of MHC I 85% and lead to the loss of cellular activity, but the histological structure of the veins were preserved well. Conclusions: The combination method with the freeze drying and irradiation can reduce the expression of MHC I distinctively. The HUV could be used as a framework to reconstruct a new arterial vessel. We consider that this lyophilize irradiated allografts has broad and glorious prospect of clinical application in the area of small caliber artery (especially in the lower extremities's artery bypass) transplantation.