葛根醇提物对糖尿病模型大鼠血脂与血管的保护作用

Protective Effects of Ethanol Extract of Pueraria lobata against Blood Lipid and Blood Vessel in Hyperglycemia Model Rats

目的:研究葛根醇提物对糖尿病模型大鼠血脂与血管的保护作用。方法:以高糖高脂饮食联合一次性腹腔注射链脲霉素以复制大鼠糖尿病模型。实验分为正常对照(等容生理盐水)组、模型(等容生理盐水)组与葛根醇提物高、中、低剂量(300、150、75 mg/kg)组。灌胃给药,每天1次,连续2个月。测定大鼠体质量、日饮水量,自动生化仪测定空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)含量;硝酸还原酶法测定一氧化氮(NO)含量;双抗体夹心固相酶免疫法测定内皮素(ET)1含量。结果:与正常对照组比较,模型组大鼠体质量显著减少,饮水量显著增加,FBG显著升高,TC、TG、LDL-C含量显著增加,HDL含量显著减少,ET-1含量显著增加,NO含量显著减少(P<0.01);与模型组比较,葛根醇提物高、中剂量组大鼠体质量显著增加,饮水量显著减少,FBG显著降低,TC、TG、LDL-C含量减少,HDL-C含量增加,ET-1含量减少,NO含量增加(P<0.01或P<0.05)。结论:葛根醇提物对糖尿病模型大鼠血脂、血管有一定改善作用,其机制可能与调节血脂平衡,维持血管活性物质动态稳定有关。

OBJECTIVE： To study the protective effects of ethanol extract of Pueraria lobata against blood lipid and blood ves- sel in hyperglycemia model rats. METHODS： Experiment is divided into normal control （constant volume physiological saline） group, model （constant volume physiological saline） group and P. lobata alcohol extraction high-dose, medium-dose and low dose （300, 150, 75 mg/kg） group. Rats were intragastrically administered, once a day for two months. High sugar and high fat diet combined with disposable intraperitoneal injection of STZ were adopted to induce hyperglycemia model. The body weight and daily water quantity were determined, and automatic biochemistry analyzer was used to determine the contents of FBG, TC, TG, HDL-C and LDL-C. Nitrate enzyme reduction method was used to determine NO content; double antibody sandwich solid-phase en- zyme immunoassay was adopted to determine the levels of ET-1. RESULTS： Compared with normal control group, body weight and the contents of HDL-C and NO were decreased significantly in model group, and daily water quantity, FBG, the levels of TC, TG and LDL-C and the content of ET-1 were increased significantly （P〈0.01）. Compared with model group, body weight, the contents of HDL-C and NO were increased significantly in ethanol extract of P lobata high-dose and medium-dose groups, and daily water quantity, FBG, the contents of TC, TG, LDL-C and ET-1 were decreased significantly（P〈0.01 or P〈0.05）. CON- CLUSIONS： The ethanol extract of P. lobata can improve blood lipid and blood vessel in hyperglycemia model rats. Its mechanism may be associated with regulating blood lipid and maintaining dynamic stability of vascular active substances.