摘要
目的:建立测定饿蚂蝗中多酚含量的方法。方法:以没食子酸为对照品,采用优化后的Folin.Ciocateau比色法(考察了测定波长、碳酸钠溶液体积、Folin.Ciocateau显色剂加入量、反应温度及时间等)测定饿蚂蝗不同提取物中多酚的含量。结果:没食予酸的质量浓度在0.001O~0.0061mg/ml范围内与其吸光度呈良好的线性关系(r=0.9994);平均加样回收率为99.99%,RSD=0.59%(n=6)。饿蚂蝗75%乙醇提取物中多酚的质量分数最高,为9.83%;其次为40%乙醇提取物,多酚质量分数为8.55%;80%乙醇提取物中多酚的质量分数最低,为0.32%。结论:采用优化后的Folin—Ciocateau比色法测定饿蚂蝗中多酚的含量,精密度和灵敏度高、稳定性和重复性好、操作简单。
OBJECTIVE: To establish the method for the content determination ofpolyphenols in Desmodium sambuse. METH- ODS : Using gallic acid as control, the content of polyphenols in different extracts of D. sambuense was determined by Folin-Cioca- teau colorimetry. The absorption wavelength, the volume of sodium carbonate, the amount of Folin-Ciocalteu regent, reaction tem- perature and time were explored. RESULTS: The linear range of gallic acid were 0.001 0-0.006 1 mg/ml(r=0.999 4) with an aver- age recovery of 99.99% (RSD=0.59%, n:6). The mass fraction of polyphenols in 75% ethanol extracts of D. sambuense was the highest, being 9.83 %; followed by 40 % ethanol extracts, being 8.55 %; that of polyphenols in 80% ethanol extract was the lowest (0.32%).CONCLUSIONS: Optimized Folin-Ciocateau colorimetry is adopted to determine the content of polyphenols in D. sam- buense, whichi is simple, stable and repeatbale with high precision and sensitivity.
出处
《中国药房》
CAS
CSCD
2013年第43期4078-4080,共3页
China Pharmacy
基金
教育部"春晖计划"立项资助合作科研项目(No.Z2011036)
贵阳市科技计划项目(No.筑科合同〔2012204〕号)