饿蚂蝗中多酚的含量测定

Content Determination of Polyphenols in Desmodium sambuense

目的：建立测定饿蚂蝗中多酚含量的方法。方法：以没食子酸为对照品，采用优化后的Folin．Ciocateau比色法（考察了测定波长、碳酸钠溶液体积、Folin．Ciocateau显色剂加入量、反应温度及时间等）测定饿蚂蝗不同提取物中多酚的含量。结果：没食予酸的质量浓度在0．001O～0．0061mg／ml范围内与其吸光度呈良好的线性关系（r=0．9994）；平均加样回收率为99．99％，RSD=0．59％（n=6）。饿蚂蝗75％乙醇提取物中多酚的质量分数最高，为9．83％；其次为40％乙醇提取物，多酚质量分数为8．55％；80％乙醇提取物中多酚的质量分数最低，为0．32％。结论：采用优化后的Folin—Ciocateau比色法测定饿蚂蝗中多酚的含量，精密度和灵敏度高、稳定性和重复性好、操作简单。

OBJECTIVE： To establish the method for the content determination ofpolyphenols in Desmodium sambuse. METH- ODS ： Using gallic acid as control, the content of polyphenols in different extracts of D. sambuense was determined by Folin-Cioca- teau colorimetry. The absorption wavelength, the volume of sodium carbonate, the amount of Folin-Ciocalteu regent, reaction tem- perature and time were explored. RESULTS： The linear range of gallic acid were 0.001 0-0.006 1 mg/ml（r=0.999 4） with an aver- age recovery of 99.99% （RSD=0.59%, n：6）. The mass fraction of polyphenols in 75% ethanol extracts of D. sambuense was the highest, being 9.83 %; followed by 40 % ethanol extracts, being 8.55 %; that of polyphenols in 80% ethanol extract was the lowest （0.32%）.CONCLUSIONS： Optimized Folin-Ciocateau colorimetry is adopted to determine the content of polyphenols in D. sam- buense, whichi is simple, stable and repeatbale with high precision and sensitivity.