四-(6-氨基己酸磺肽基)铝(Ⅲ)氯酞菁/牛血清白蛋白复合物的光物理性质

The photophysical properties of tetra-carboxyl-aminohexanoic-sulfonyl phthalocyanine aluminium(Ⅲ) chloride/bovine serum albumin conjugates

采用荧光光谱法和紫外光谱法比较研究了新型含空间链四-(6-氨基己酸磺肽基)铝氯酞菁(AlPc(SO2NHC5H10COOH)4)及AlPc(SO2NHC5H10COOH)4/牛血清白蛋白(BSA)复合物的光物理性质。与无空间链四羧基铝氯酞菁(AlPc(COOH)4)相比较,AlPc(SO2NHC5H10COOH)4不易聚集,荧光强度增强,荧光寿命增长。当AlPc(SO2NHC5H10COOH)4和AlPc(COOH)4分别与BSA形成复合物后,与相应的自由酞菁相比,AlPc(COOH)4/BSA和AlPc(SO2-NHC5H10COOH)4/BSA复合物的紫外光谱红移,荧光光谱猝灭,其荧光猝灭原因为静态猝灭。由于空间链效应,AlPc(SO2NHC5H10COOH)4与BSA之间的作用明显强于AlPc(COOH)4与BSA之间的作用。

The purpose of this study was to investigate the photophysical properties of 3rd generation photosensi- tizer using in photodynamic therapy. The photophysical properties of tetra-carboxyl-aminohexanoic-sulfonyl phthalocyanine aluminium chloride （A1Pc （SO2 N HC2 H10 CO0 H） 4 ）-bovine serum albumin （BSA） bioconj ugate was studied by UV-Vis and fluorescence spectroscopic method. The results indicated A1Pc （SO2 NHC5 H10COOH）4 exhibited lower aggregation, higher fluorescence intensity and longer lifetime compared with tetra-carboxyl phthaloeyanine aluminium chloride （A1Pc（COOH）4） which may due to the spacer hindrance effect. When A1Pc（SO2NHC4H10COOH）4 and A1Pc（COOH）4 conjugates with BSA, compared with the corre- sponding free phthalocyanine, the UV-Vis spectra of A1Pc（COOH）4/BSA and A1Pc（SO2NHCsH10COOH）4/ BSA was red shift and fluorescence spectra was quenched. Furthermore, A1Pc（SO2NHCsHloCOOH）4 conju- gate also showed stronger combining interaction with bovine serum albumin than that of A1Pc（COOH）4 due to the spacer hindrance effect.