MRE11沉默对肝癌耐药细胞BEL7402/5-FU增殖和凋亡的影响

Effect of MRE11 gene silence on the proliferation and apoptosis of multidrug-resistant hepatocellular carcinoma cell line BEL7402 / 5-FU

目的:观察shMRE11对肝癌耐药细胞BEL7402/5-FU细胞增殖和凋亡的影响,初步探索MRE11与肝癌发生发展的关系。方法:采用阳离子脂质体法将shMRE11干扰质粒转染BEL7402/5-FU细胞,Real-time PCR及Western blot检测沉默效率;MTT法检测转染前后BEL7402/5-FU细胞增殖情况;AnnexinV-FITC/PI双染法检测MRE11基因沉默对BEL7402/5-FU细胞凋亡的影响。结果:Real-time PCR及Western blot检测结果显示MRE11 mRNA及蛋白水平的沉默效率分别为78.0%、56.1%。MTT结果显示,shMRE11转染BEL7402/5-FU细胞后,shMRE11实验组与对照组相比细胞增殖速度减慢(P<0.05)。AnnexinV-FITC/PI双染法检测结果显示shMRE11实验组的细胞凋亡指数增高,与对照组比较差异有统计学意义(P<0.05)。结论:shMRE11干扰质粒能够抑制BEL7402/5-FU细胞增殖,促进细胞凋亡。

Objective To investigate the effect of shMREll on the proliferation and apoptosis of muhidrug-resistant hepatocellular carcinoma cell line BEL7402/5-FU. Methods shMRE11 interfering plasmid was transiently transfected into BEL7402/5-FU ceils via cathodolyte liposome transfection method. The silence efficiency was evaluated by Real-time PCR and Western blot. The proliferation of BEL7402/5-FU cells was determined by MTT. The apoptosis of BEL7402/5-FU cells was measured by AnnexinV-F[TC/PI double staining methods. Results The efficiency of RNA interference for MREll was 78.0% on mRNA level and was 56.1% on protein level. In shMRE11 group, the proliferation rate was slower and the apoptosis index was higher than those in control group （all P 〈 0.05）. Conclusions The shMREll interfering plasmid could effectively inhibit the proliferation and promote the apoptosis of BEL7402/5-FU cells.