贯叶连翘的组织培养和快速繁殖

Tissue culture and rapid regeneration of Hypericum perforatum

目的 :探索贯叶连翘外植体组织培养的适合培养基配方。方法 :将贯叶连翘种子发芽产生的根、带节茎段、叶作为外植体 ,培养在含不同种类及浓度激素的 Murashige and Skoog ( MS)基本培养基上 ,诱导愈伤组织、芽和根的形成 ,并进行试管苗的移栽。结果 :不同激素配比和不同种类外植体对愈伤组织和芽的形成有较大区别。 2 ,4-二氯苯氧乙酸 ( 2 ,4- D)有利于促进贯叶连翘愈伤组织的形成 ;在细胞分裂素中 ,6 -苄基氨基嘌呤 ( 6 - BA)促进生芽的作用很明显 ,而激动素 ( KT)、玉米素 ( ZT)则不明显 ;α-萘乙酸 ( NAA) 1mg/ L 与 6 - BA0 .2 m g/ L 的组合有利于贯叶连翘的生根。 结论 :含有 2 ,4- D的 MS培养基有利于愈伤的诱导 ,外植体根最适合丛生芽的快速诱导。

Objective: To study the proper medium for tissue culture of the explants from Hypericum perforatum . Methods: The root, stem with node and leaf of the Hypericum perforatum seedling were used as explants, and cultured in basic Murashige and Skoog (MS) medium supplemented with different kinds and different concentrations of hormones. The callus, buds and roots were induced. The cultivation of the tube seedling were carried out. Results: Different hormones and explants had different effects on the induction of callus and the formation of buds. 2,4 D could promote the formation of the callus of Hypericum perforatum . Among cytokinins, 6 BA had great effect on the formation of buds, but the effects of KT and ZT were insignificant. The combination of NAA 1 mg/L and 6 BA 0.2 mg/L was found most effective in promoting the rooting of Hypericum perforatum . Conclusion: The results indicate that the callus is easy to be induced when MS medium supplemented with 2,4 D. The root of explants is most suitable for the rosette bud rapid induction.