HPLC法测定酸模属植物根中蒽醌类成分的含量

Content determination of three kinds of anthraquinones in the root of Rumex by HPLC

目的 :考察酸模属植物根中蒽醌类成分的含量 ,以评价其药用价值。 方法 :采用 HPL C法对 5种酸模属植物根及 3个不同产地羊蹄根中的蒽醌类成分进行含量测定。结果 :该属 5种植物根中蒽醌类成分的含量范围分别为 :大黄素 0 .0 5 1%～0 .30 3%、大黄酚 0 .0 95 %～ 0 .32 0 %、大黄素甲醚 0 .0 2 5 %～ 0 .142 % ;3个不同产地羊蹄根中的蒽醌类成分的含量范围分别为 :大黄素 0 .0 5 1%～ 0 .12 5 %、大黄酚 0 .12 7%～ 0 .193%、大黄素甲醚 0 .0 49%～ 0 .0 96 %。结论 :巴天酸模及羊蹄根中的蒽醌类成分含量相近 ,从化学上支持两者都作为“羊蹄”入药的传统用法 ;不同产地及生境的羊蹄根中 ,蒽醌类成分含量存在差异 ,3种蒽醌类成分以南京产羊蹄为最高 ;金不换及酸模根中蒽醌类成分较高 ,而齿果酸模中各成分含量均较低。

Objective:To investigate the contents of anthraquinones in the root of Rumex , and to evaluate its pharmaceutical value. Methods: HPLC method was applied to measure the contents of anthraquinones in the root of 5 kinds of Rumex and 3 intraspecies of R. japonicus. Results: The contents of emodin, chrysophanol, physcion ranged from 0.051% 0.303%, 0.095% 0.320%, 0.025% 0.142% in the root of 5 kinds of Rumex , and ranged from 0.051% 0.125%, 0.127% 0.193%, 0.049% 0.096% respectively in 3 intraspecies of R. japonicus. Conclusion: Both R.patientia and R.japonicus can be employed as ‘Yangti’ clinically as antibiotics for their anthraquinone contents are almost the same. Contents of anthraquinone in R. japonicus from different place and under different enviroment are different. Contents of 3 anthraquinones in R.japonicus (Nanjing) are the highest, and those in R.madaio and R.acetosa are next to them, but less in R.dentatus . [