摘要
目的:探讨抑制剂PI-103对786-O细胞株PI3K/Akt/mTOR信号通路的影响。方法:本文建立PI3K抑制剂作用786-O细胞模型,CCK-8法检测PI-103的IC50值,Western blot法检测PI-103作用后细胞内pAkt蛋白表达水平。结果:786-O细胞72 h的IC50为(9.06±0.06)μmol/L,在此浓度下,细胞内p-Akt蛋白的表达水平显著降低(P<0.01)。结论:PI-103通过抑制PI3K来调控PI3K/Akt/mTOR信号通路下游分子,此信号通路在肾细胞癌发生发展中起着重要作用,为肾细胞癌的分子靶向治疗提供理论依据。
Objective： To explore the effect of PI3K/Akt/mTOR signal pathway in 786-O cell lines by inhibitor PI-103. Methods： Establish PI3K inhibitor 786-O cell model,CCK-8 assay IC50 values of PI-103 and Western blot assay p-Akt protein expression level. Results： 786-O cells 72 h IC50 was（ 9. 06 ± 0. 06） μmol/L,p-Akt protein expression level in the cells was significantly lower（ P 0. 01）. Conclusion： PI-103 regulates the PI3K/Akt/mTOR signal pathway through inhibition of PI3K. This signal pathway plays an important role in renal cell carcinoma,which can provide a theoretical basis for molecular targeted therapy of renal cell carcinoma.
出处
《河南医学研究》
CAS
2013年第5期649-651,共3页
Henan Medical Research
