白细胞介素22治疗变应性鼻炎小鼠的实验研究

The role of interleukin-22 in the treatment of allergic rhinitis in mice

目的：探讨鼻腔局部给予重组小鼠白细胞介素22（IL-22）对小鼠变应性鼻炎免疫应答的调节作用。方法将24只6～8周的雌性BALB/c小鼠随机分为3组，每组8只，分别为对照组、模型组和IL-22组。以卵白蛋白（ovalbumin/OVA）为致敏原，氢氧化铝凝胶为佐剂制备变应性鼻炎小鼠模型，于激发前给予小鼠鼻腔局部滴注重组小鼠IL-22。观察小鼠变应性鼻炎症状改善情况，并检测三组小鼠鼻黏膜局部嗜酸性粒细胞侵袭情况、外周血清中OVA-特异性IgE（OVA-sIgE）浓度及Th1、Th2细胞因子的表达情况。结果重组小鼠IL-22干预的变应性鼻炎小鼠症状较模型组缓解，鼻腔黏膜局部嗜酸性粒细胞侵袭减少， OVA-sIgE浓度降低，Th2型细胞因子IL-4和IL-5浓度下降。差异均具有统计学意义（P〈0.05）。Th1型细胞因子IFN-γ浓度在IL-22治疗组和模型组间差异无统计学意义。结论鼻腔局部给予重组小鼠IL-22可以抑制变应性鼻炎小鼠的免疫反应，它有望成为治疗变应性鼻炎的一种新方法。

Objective Explore regulatory effect of immune response in mice with allergic rhinitis by nasal topical administration of recombinant mouse interleukin 22（IL-22）.Methods 6 to 8 weeks,24 female BALB/c mice were randomly divided into three groups,each group of 8,respectively,for control group,the model group and IL-22 treatment group.Allergic mice were prepared with chicken ovalbumin as allergen and aluminium hydroxide gel as immune adjuvant.Recombinant mouse IL-22 was administrated into the nostril.Observe the symptoms of allergic rhinitis mouse and detected nasal mucosa local eosinophil invasion,concentration of peripheral serum ovalbumin-specific IgE（OVA-sIgE） and Th1,Th2 cytokine expression.Results Symptoms of recombinant mouse IL-22 intervention allergic rhinitis mice were relieved compared to the model group.Reduce local eosinophil invasion of the nasal mucosa,concentration of OVA-s IgE decrease and levels of Th2 cytokines IL-4 and IL-5 levels drop down.Differences were statistically significant （P〈0.05）.The differences of Th1 cytokines IFN-γ concentration between IL-22 group and model group have no statically significance.Conclusion Nasal locally administered recombinant mouse IL-22 can inhibit the allergic reaction in mice.IL-22 is expected to become a new method for treatment of allergic rhinitis.