切应力作用下联合培养的血管内皮细胞血管假性血友病因子的含量变化

The changes of von Willebrand factor content in endothelial cells co-cultured with vascular smooth muscle cells under shear stress

目的 :研究切应力对与血管平滑肌细胞联合培养的内皮细胞的抗凝血和粘附的影响。方法 :对与平滑肌细胞联合培养的内皮细胞施加生理范围的切应力 ,用免疫组化及图像分析法检测内皮细胞的血管假性血友病因子 (v WF)含量。 结果 :联合培养的内皮细胞 v WF的变化与切应力大小及作用时间有关 ,随切应力增大 ,胞内 v WF含量逐渐减少 ,且切应力作用的第 1小时减少的最快。结论 :生理范围的切应力作用 ,可能减少与平滑肌细胞联合培养的内皮细胞的 v WF表达 ,或促使 v WF短时释放加快 ,有利于增强抗凝血功能及内皮细胞的粘附。

Objective: To investigate the effect of the shear stress on anti coagulation and adhesion ability of ECs co cultured with VSMCs. Methods: ECs co cultured with VSMCs were exposed to physiological laminar shear stress, vWF in ECs were detected by immunohistochemical method and computer image analysis.Results: The changes of vWF in ECs was correlated with the shear stress. The larger the shear stress was, the smaller the quantity of vWF, especially in the 1st hour. Conclusion: The physiological laminar shear stress probably decrease the expression of vWF in Ecs or accelerate the release of vWF in ECs in short time, which facilitate the enhancing of anti coagulation and the adhesion ability of ECs co cultured with VSMCs. [