16S rRNA基因序列分析法鉴定病原细菌

Identification of pathogenic microorganism by sequencing 16S rRNA gene

目的:运用16S rRNA基因序列分析法鉴定14种细菌,为该方法的临床应用奠定基础。方法:提取细菌DNA,采用通用引物PCR扩增16S rRNA基因片段并测序。将测序结果用Blastn在线软件在Nucleotide数据库中进行序列同源性比对,根据序列同源性鉴定病原细菌。结果:12种细菌可以鉴定到"种",2种细菌可以鉴定到"属"。结论:16SrRNA基因序列分析是一种有效的病原细菌鉴定方法。

Objective： To identify 14 bacteria by sequencing the 16S rRNA gene and establish the basis for clinical application in the future. Methods： DNA samples of the 14 bacteria were extracted. The 16S rRNA genes were amplified by PCR and sequenced with common primers. The sequences of the 16S rRNA genes were aligned by online software Blastn in nucleotide database. The bacteria were identified according to the homology of their 16S rRNA genes. Results： Twelve bacteria were classified to species, the other 2 bacteria were classified to genus. Conclusion： 16S rRNA gene sequence analysis is useful in identifying pathogenic bacteria.