高压氧联合^(32)P胶体对大鼠种植瘤生长及血清VEGF表达的影响

Effect of Hyperbaric Oxygen in Combination with ^(32)P-colloid on Implanted Tumor Growth and Serum VEGF Expression in Rats

目的探讨高压氧(HBO)治疗后立即行放射性药物32P胶体治疗对大鼠种植瘤生长及血管内皮生长因子(VEGF)表达的影响。方法完成大鼠乳腺癌Walker-256细胞种植性肿瘤模型的制作1周后,将80只实验动物随机分为A、B、C、D4组,即对照组、高压氧治疗组(HBO组)、32P胶体治疗组、胶体32P后高压氧治疗组(联合治疗组),每组20只。A、C组通过瘤体内直接注射法分别给予相同体积的生理盐水、32P胶体;B组注入生理盐水后连续高压氧治疗14天;D组注射32P胶体后行高压氧治疗持续14天。测定各组肿瘤7、12、20天时的体积,并计算生长率;通过免疫组化技术检测肿瘤组织的血管内皮生长因子(VEGF)的表达。结果 A、B、C、D组第12、20天时的肿瘤生长率分别为232.12%±67.52%、878.64%±425.36%;208.23%±78.78%、845.02%±632.67%;145.65%±55.37%、502.06%±312.96%;104.18%±43.75%、113.86%±42.38%,联合治疗组明显小于其他各组(P<0.05)。A、B、C、D组肿瘤组织中VEGF阳性表达率分别为90.0%(18/20)、80.0%(16/20)、30.0%(6/20)、10.0%(2/20),A、B组之间无统计学差异(P>0.05),A、B组的阳性表达率明显高于C、D组(P<0.05),A组尤为明显,C、D组之间存在明显差异(P<0.05)。结论高压氧联合放射性药物32P胶体可显著抑制大鼠种植瘤的生长,其机制可能与抑制VEGF的表达、肿瘤血管新生,从而抑制癌细胞增殖有关。

Objective To investigate the effect of 32p _ colloid therapy immediately after hyperbaric oxygen treatment on implanted tumor growth and serum VEGF expression in rats. Methods Immediate Walker- 256 cancerous ascetic injection was used to make a rat tumor - bearing model, then the l w later 80 rats were randomly divived into control group （ A group） , hyper - baric oxygen group （ B group）, 32 p _ colloid group （ C group） , cooper - ration of hyperbaric oxygen and 32 p _ colloid group （ D group） , with 20 in each group. Ratsin A and C group by the direct injection of tumors in vivo were respectively given the same volume of saline and 32p _ colloid. Ratsin B group were treated with hyperbaric oxygen therapy for 14 days after the injection of saline. Ratsin D group were given hyperbaric oxygentherapy for 14 days after the injectin of 32p _ colloid. Ratsin Every group were measured tumor volume on 7th, 12th and 20th day to calcu- late its growth rate. The vascular endothelial growth factor（VEGF） was performed by immunohistochemistry method. Results The tumor growth rates of the 12th and 20th day in A, B, C and D groups were 232.12% 67.52% and 878.64% ± 425.36% ,208.23% _＋ 78.78% and 845.02% 632.67% ,145.65% ±55.37% and 502.06% 312.96%,104.18% 43.75% and 113.86% 42.38% respeltirely. The rate of D group was significantly less than those of A,B and C groups（P 〈0.05）. The rate of C group was obviously less than those of A and B groups（ P 〈 0.05 ）. There was no distinguished difference between the rates of A and B groups（ P 〉 0.05 ）. The VEGF positive expression rates in A,B,C and D groups were 90. 0% （18/20）, 80.0% （16/20）, 30.0% （6/20）, 10.0% （2/20）. There was no sig- nificant difference between A and B groups（ P 〉 0.05）. The positive expression of A and B groups was significantly higher than that of C and D groups（P 〈 O. 05）. There was distinguished difference between C and D groups（ P 〈 0.05 ）. Conclusion The tumor growth was inhibited significantly after the treatment of hyperbaric oxygen in combination with 32p _ colloid. The mechanism may being the expression and the angiogenesis of blood vessel being inhibited, then the proliferation of cancer cells being inhibited.