PEDF、IL-6与2型糖尿病视网膜病变的临床研究

Correlation between PEDF,IL-6 and Type 2 Diabetic Retinopathy

目的探讨血清色素上皮衍生因子(pigment epithelium derived-factor,PEDF)、白介素-6(Interleukin-6,IL-6)在2型糖尿病视网膜病变中的临床意义。方法选取118例2型糖尿病患者为实验组,根据免散瞳眼底检查结果,实验组分为无糖尿病视网膜病变(NDR)组48例、单纯型糖尿病视网膜病变(BDR)组40例、增殖型糖尿病视网膜病变(PDR)组30例,对照(NC)组为健康人30例。采用酶联免疫吸附法和电化学发光法分别检测各组血清样本PEDF、IL-6含量,并整理临床数据,用SPSS 17.0软件进行单因素方差分析和直线相关性分析。结果 NC组、NDR组、BDR组、PDR组的血清PEDF含量分别为281.23±90.81、184.07±10.36、79.68±11.04、53.84±6.28μg/L;IL-6含量分别为3.33±1.49、8.99±0.80、15.81±1.49、18.62±1.04pg/ml。实验组血清中PEDF、IL-6含量与NC组比较差异均有统计学意义(F=73.65,P<0.01;F=177.17,P<0.01)。NDR组、BDR组、PDR组血清PEDF含量有依次减低的趋势,血清IL-6含量有依次增加的趋势。血清PEDF含量与DR的严重程度呈负相关(r=-0.941,P<0.01);血清IL-6含量与DR的严重程度呈正相关(r=0.930,P<0.01)。结论 PEDF、IL-6在DR的发生、发展中有重要作用,可为临床有效防治DR提供依据。

Objective To investigate the clinical significance of serum pigment epithelium derived -factor（PEDF） ,interleukin -6 （ IL - 6） in type 2 diabetic retinopathy. Methods We selected 118 pitients with type 2 diabetes as the experimental group. According to non- mydriatic fundus examination, the experimental group was divided into non- diabetic retinopathy（NDR,48cases） , background dia- betic retinopathy（BDR,40cases） , prolifera - tire diabetic retinopathy （ PDR, 30cases）. Other 30 normal persons were as controls （ NC ）. The levels of PEDF, IL -6 were determined by ELISA and ECLIA. Data were evaluated using analysis of SPSS17.0 software. Statistical comparison was performed by single - factor analysis of variance and Linear correlation analysis. Results The level of PEDF in the serum from NC, NDR,BDR and PDR were 281.23 ± 90.81,184.07 ±10.36,79.68± 11.04,53.84 ±6. 281~g/L. The level of IL- 6 in the ser- um from NC,NDR,BDR and PDR were 3.33± 1.49,8.99 ± 0.80,15.81 ＋ 1.49,18.62 ± 1.04pg/ml. Compared with the control, the level of PEDF and IL - 6 in the serum of experimental groups was different significantly（ F = 73.65, P 〈 0.01 ; F = 177.17, P 〈 0.01 ）. The level of PEDF in the serum of the NDR, BDR and PDR reduced significantly, and the level of IL - 6 in the serum of the NDR,BDR and PDR increased significantly. The serum PEDF and DR showed a significant negative correlation（r= -0. 941 ,P 〈0. O1 ）. The serum IL - 6 and DR showed a significant positive correlation（ r = 0. 930,P 〈 0.01 ） Conclusion The PEDF and IL - 6 play an important role in the development in DR, which can provide clinical evidence for the prevention and treatment of DR.