针灸对MNNG诱导大鼠癌前病变胃黏膜病理及血清CEA影响观察

Acupuncture and moxibustion on pathologic and serum CEA changes in rats with precancerous lesion induced by MNNG

目的:研究针灸预处理对Wistar大鼠癌前病变胃黏膜干预作用和对血清CEA的影响。方法:将50只Wistar雄性大鼠随机分为4组,其中空白对照组10只;其余40只大鼠自由饮用100μg/mL的N-甲基-N’-硝基-N-亚硝基胍(MNNG)6个月诱发大鼠胃癌模型,模型组20只;艾灸组和针刺组均10只,在造模3个月以后对足三里和梁门穴进行相应的治疗干预。处死大鼠后取胃黏膜进行HE染色,光镜下观察各组大鼠胃黏膜形态,并采用IPP 6.0图像分析软件比较各组胃黏膜细胞核质比。股动脉留取大鼠血液,ELISA法检测血清CEA。结果:光镜结果显示,艾灸和针刺预处理组大鼠胃黏膜相对于模型组大鼠炎症和异型性等变化较轻;模型组核质(面积)比为1.51±0.69,明显高于空白对照组0.85±0.33,P<0.001;艾灸组为0.70±0.29,小于模型组,P=0.006 4;针刺组为0.99±0.43,亦小于模型组,P=0.040 0。酶联免疫吸附实验(enzyme-linked immuno sorbent assay,ELISA)结果显示,模型组血清CEA为(372.42±137.61)ρg/mL,较空白组(243.13±59.07)ρg/mL升高,P=0.007 2;艾灸组为(285.63±122.14)ρg/mL,较模型组降低,P=0.044 5;针刺组为(302.89±110.02)ρg/mL,较模型组降低,但差异无统计学意义,P=0.402 0。结论:针灸预处理能够改善MNNG导致的大鼠胃黏膜癌前病变的组织学表现和降低血清CEA浓度。

To investigate the function and mechanisms of pretreatment of acupuncture and moxibustion in Wistar rats with precancerous lesions. METHODS： Fifty male rats were randomly divided into 4 groups. Blank control group had 10 rats. Rats in other groups taken 100μg/mL of N-methyl-N-nitro-N-nitrosoguanidine （MNNG） freely in 6 months. Model group had 20 rats. Moxibustion group and acupuncture group had 10 each. On the third month,the rats in moxibustion and acupuncture groups received relevant intervention on Zusanli and Liangmen acupoints. After the rats were executed, the gastric mucosas were processed by HE stain and observed under microscope. The nucleoplasm ratio of gastric mucosa cells were analysised under IPP 6.0 software. The ser um CEA of each group was measured by ELISA method. RESULTS： Compared to model group, the gastric mucosa in moxibustion group and acupuncture group showed obvious improvements in inflammatory reaction, dysplasia ect. Compared with the nucleoplasm ratio of blank group 0. 85±0.33 ,the model group had higher ratio of 1.51±0. 69（P〈0. 001）. The moxibustion group was 0. 70± 0. 29,which was less than that of model group（P=0. 006 4）. The acupuncture group was 0. 99±0. 43,which was less than that in model group too （P=0. 040 0）. Serum CEA of model group was （372. 42±137. 61） μg/mL,which was higher than that of blank group （243. 13：t：59.07）μg/mL （P=0. 007 2）. The moxibustion group was （285.63±122. 14） μg/mL,which was lower than that of model group （P=O. 044 5）the acupuncture was （302.89±110. 02） μg/mL,which was lower than that of model group but there was no statistical difference（P= 0. 402 0）. CONCLUSION： Pretreatment of acupuncture and moxibustion in Wistar rats with precancerous lesions caused by MNNG have showed obviously better outcomes in pathological changes and serum CEA.