火木层孔菌发酵菌粉及其组分对小鼠移植性肝癌H22的体内生长作用

Effects of dry matter of culture broth from Phellinus igniarius submerged fermentation and its fractions on mice hepatoma 22

采用肝癌H22荷瘤小鼠的肿瘤模型,对火木层孔菌(桑黄)Phellinus igniarius发酵菌粉及其各提取物组分的体内抗肿瘤活性进行评价。结果表明,火木层孔菌发酵菌粉及其各提取物组分对荷瘤小鼠肝癌H22都具有一定的抗肿瘤作用并能延长荷瘤小鼠生存期,其中,火木层孔菌发酵菌粉(1,000mg/kg/d)及其组分I(I多糖组分)(360mg/kg/d)具有较为显著的抗肿瘤作用,抑瘤率分别为33.5%和40.3%。组织病理学研究结果表明在菌粉及其多糖组分作用后,肿瘤细胞坏死细胞明显增多,免疫组化检测表明火木层孔菌发酵菌粉及其多糖组分能明显的降低瘤组织中Bcl-2基因蛋白的表达,提高小鼠瘤组织中的Bax基因蛋白的表达。火木层孔菌发酵菌粉及其多糖组分具有较好的体内抗肿瘤活性。

The inhibitory effects of dry matter of culture broth （DMCB） from Phellinus igniarius submerged fermentation and its fractions were evaluated with the mice transplanted Hepatoma 22 in vivo. The results showed that both of DMCB and its fractions exhibited anticancer effect and increased the life span. The potency of DMCB and its fraction II （polysaecharides） seems to be better than that of the other two fractions. The inhibitory rate at the high dose of DMCB （1,000mg/kg/d） and fraction II （360mg/kg/d） was 33.5% and 40.3% respectively. The histopathological results showed that necrosis in H22 cells was induced in both DMCB and fraction II treatment groups, and immunohistochemieal detectionindicated the decrease in Bcl-2 expression and the increase in Bax expression in the cancerous tissue of the mice, DMCB from Phellinus igniarius submerged fermentation and its polysaccharides fraction possess potent antitumor activity in vivo.