反相高效液相色谱法测定食用菌中7种有机酸的研究

Determination of seven organic acids in edible fungi by reversed-phase high performance liquid chromatography

利用反相高效液相色谱法同时分析食用菌中7种有机酸,优化后的色谱条件为:Green ODS-AQ柱(4.6mm×250mm,5μm),流动相为10mmol/L的磷酸二氢钾(pH2.8),流速为1.0mL/min,柱温30℃,检测波长为210nm,进样量10μL。该方法精密度、重复性、稳定性实验中7种有机酸的RSD值均小于5%,加样回收率在94.6%–99.3%之间;该方法简便,可应用于食用菌中7种有机酸的检测。运用建立的方法测定了8种食用菌中7种有机酸成分,结果发现不同食用菌中有机酸的种类和含量均差异显著。

A method for determination of seven organic acids in edible fungi using reversed-phase high performance liquid chromatography （HPLC） was improved. The HPLC system was as follow： Green ODS-AQ column was used at 30℃ and mobile phase was 10mmol/L KH2PO4 （pH2.8） with flow rate of 1.0mL/min. The UV detection wavelength was 210nm and sample volume was 10μL. The results indicated that seven kinds of organic acid RSD value were less than 5% in precision, repeatability and stability experiment of this method, and sample recovery rate was between 94.6% to 99.3%. The method was simple which could be applied to determining organic acids in edible fungi. Seven organic acids in eight kinds ofmushrooms were analyzed under favorable conditions. Results showed that the variety and content of organic acids in these mushrooms are quite different.