摘要
【摘要】目的检测内质网相关分子C/EBP同源蛋白(C/EBPhomologyprotein,CHOP)在缺血再灌注大鼠脑内的表达变化及参芎注射液对CHOP的影响,以期为参芎注射液的脑保护作用提供理论依据。方法144只雄性SD大鼠按随机数字表法分为三组:假手术组、手术组、参芎组。采用线栓法制作大脑中动脉缺血再灌注模型,分别于再灌注后6、12、24、72h时相点处死动物,用免疫组化学及逆转录聚合酶链反应法分别检测脑缺血区CHOP蛋白水平及mRNA水平;末端标记法原位检测神经细胞凋亡。结果CHOPmRNA及蛋白水平在再灌注后均有升高,分别在再灌注后12、24h时相点达到高峰;在缺血再灌注模型中,神经细胞凋亡的数量随再灌注时间延长而增加,24h时相点到达高峰;在再灌注后各个时间点参芎干预组凋亡细胞数均低于手术组(P〈0.01);再灌注后6、12、24、72h参芎干预组CHOPmRNA及蛋白水平明显低于相应时间点的手术组大鼠(P〈0.01)。结论参芎注射液可能通过抑制脑缺血再灌注后内质网应激诱导的CHOP的表达进而减少神经细胞凋亡,发挥神经保护作用。
[ Abstract] Objective To investigate the expression of C/EBP homology protein (CHOP) in rat brain tissue after focal cerebral ischemia-reperfusion, as well as to observe the influence of the shenxiong in- jection on the expression of CHOP. Methods One hundred forty four male rats were randomly divided into three groups: the sham-operation group, operation group, sbenxiong group. Focal cerebral ischemia and reperfusion rat models were established by using suture. Zea Longa method was introduced to evaluate neu- rologic behavioral changes. The levels of mRNA and protein of CHOP were measured with methods of immu- nohistochemistry and reverse transcription polymerase chain reaction. The neuronal apoptosis was detected by the method of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling. Results The expression of CHOP was up-regulated in per-infarction after cerebral ischemia-reperfusion in rats. CHOP mRNA and protein levels peaked at the 12th h and 24th h after reperfusion, respectively. The apop- tosis cell counting increased gradually after cerebral ischemia-reperfusion and peaked at the 24th h after reperfusion. Compared with the saline control group, treatment with shenxiong injection could reduce the neuronal apoptosis at the 6th, 12th, 24th, and 72nd h after reperfusion ( P 〈 0. 01 ). Compared with the saline control group, treatment with shenxiong injection could decrease CHOP mRNA and protein expression at the 6th, 12th, 24th, and 72nd h after reperfusion ( P 〈 0. 01 ). Conclusions Shenxiong Injection may prevent neurocyte from apoptosis by inhibition of the expression of CHOP induced by endoplasmic reticulum stress.
出处
《中国医师杂志》
CAS
2013年第10期1338-1341,共4页
Journal of Chinese Physician
关键词
脑缺血
病理学
再灌注损伤
预防和控制
内质网
代谢
人参
药理学
ill芎嗪
药理学
注射剂
细胞凋亡
Brain ischemia/pathology
reticulum/metabolism
GINSENG A/pharmacology
rApoptosisReperfusion injury/prevention & control
Endoplasmicpharmacology
INJECTIO