高迁移率族蛋白1在卵巢子宫内膜异位症中的表达及意义

The expression and siotmificance of high mobility group protein boxl in ovarian endometriosis tissue

【摘要】目的研究高迁移率族蛋白1（HMGBl）在卵巢子宫内膜异位症中的表达，为子宫内膜异位症的早期诊断、治疗及预后提供新的思路。方法采用RT—PCR和western—blotting检测HMGBImRNA和HMGBl蛋白在69例卵巢子宫内膜异位症患者异位子宫内膜和在位内膜及69例非子宫内膜异位症患者的在位内膜中（对照组）的表达情况。结果HMGBlmRNA在卵巢异位子宫内膜、在位内膜和非异位症的在位内膜均有表达，其表达灰度值分别为：0．5438±0．0565、0．3016±0．0614、0．1536±0．0444；HMGBl蛋白在三组内膜中表达的灰度值分别为：0．8399±0．0287、0．4010±0．0643、0．1999±0．0716。三组HMGBlmRNA、HMGBl蛋白的表达水平比较差异均有统计学意义（F=8．756，P〈0．05）。HMGBl在各组不同月经时期表达水平比较差异均无统计学意义（P〉0．05）。卵巢子宫内膜异位症患者临床分期高级别异位内膜中HMGBl表达水平高于低级别者（t=10．28，P〈0．01），与肿瘤大小、患者年龄无明显相关（P〉0．05）。结论HMGBl在卵巢子宫内膜异位、在位内膜中呈高表达，且临床分期级别越高其表达增强，其可能在卵巢子宫内膜异位症的发生、发展中起着重要的作用，可作为子宫内膜异位症的生物标记物。

[ Abstract ] Objective To investigate the expression of high mobility group protein boxl （ HMGB1 ） in ovarian endometriosis tissue and to provide new idea for early diagnosis, treatment and prognosis of endo- metriosis. Methods The levels of HMGB1 in ectopic endometrium and eutopic endometrium of 69 patients with ovarian endometriosis and endometrium of 69 cases with non-endometrioses were detected by reverse transcription-polymerase chain reaction （RT-PCR） and Western-blotting. Results HMGB1 was expressed in all the three groups （ ectopic endometrium eutopic endometrium endometrium with non - endometrioses）. The relative expression level of HMGB1 mRNA was 0. 54383 4± 0. 0565, 0. 3016 4± 0. 0614, and 0. 1536 4± 0. 044n., respectively （ F = 377. 923, P 〈 0. 0001 ）. The relative of HMGB1 protein was 0. 83990 ＋ 0. 12869, 0. 40100 4. 0. 16425, and 0. 19986 ＋ O. 08162, respectively （ F = 185. 625, P = 0. 0000）. The expression level of HMGB1 was related to clinical stages （ t = 10. 28, P 〈0. 01 ） ; however, it was not ob- viously related to age, the diameter of lump, and course of disease （ P 〉 0. 05）. Conclusions Over ex- pression of HMGB1 in ovary endometriosis indicates that HMGB1 plays a significant role in occurrence and development of ovary endometriosis. The expression of HMGB1 in endometriosis eutopic endometrium was much higher than that of non-endometrioses endometrium, which supports the eutopic endometrium deter- minism. The overexp^ssion of HMGBI was consistent with clinical stage, which proves that HMGB1 maybe be tighdy associated with the severity of the disease and to likely become biomarker.