兔骨骼肌钝挫伤恢复过程中碱性成纤维细胞生长因子的变化及按摩的干预作用

Changes in basic fibroblast growth factor during the healing process of rabbit skeletal muscle contusion and intervention effect of massage

目的:研究新西兰兔股四头肌经重物外力损伤后,在肌组织损伤修复过程中,按摩对肌肉组织的病理修复、超声影像学观察以及对碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的影响,探讨按摩促进肌肉损伤修复的作用机制。方法:健康成年雄性新西兰大白兔32只,体质量(2.0±0.5)kg,按随机抽样法分为正常对照组(A组,n=4)和模型组,模型组分为按摩前对照组(B组,n=4)、自然恢复组(C组,n=12)和按摩组(D组,n=12)。A组实验动物不作任何处理,作为正常对照;B、C、D组实验动物用自制打击器制备兔右后肢股四头肌损伤模型。C组不予按摩,D组于制模后第5天开始按摩。C、D组于伤后第7、14天及第21天各取4只实验动物股四头肌样本,免疫组化学方法检测bFGF蛋白表达,HE染色观察其组织病理变化,微泡超声影像技术检测制模后第7、14天的组织坏死情况。结果:按摩干预后,C组和D组比较,第7天bFGF表达量D组>C组(P=0.016),而在制模后第14、21天bFGF表达量C组均﹥D组(P=0.000,P=0.020)。超声微泡造影及HE染色显示,D组与C组比较,损伤部位较轻,血供较丰富,坏死区明显减轻且血管恢复较快。结论:按摩可有效防止肌细胞的萎缩、退变及坏死,为损伤区域提供丰富血供,促进血管生成,其利于损伤修复及新生血管形成的机制可能与bFGF有关。

Objective：To investigate the effect of massage on quadriceps femoris repair after injury by external force with the histologi- cal observation and ultrasound imaging observation as well as the influences of basic fibroblast growth factor （bFGF） in New Zealand rabbits so as to explore the possible molecular mechanisms of massage in repair of muscle injury. Methods.Thirty-two New Zealand white rabbits,weighing （2.0 ± 0.5） kg, were randomly divided into 4 groups：normal control （group A, n=4）, control group before the massage （group B, n=d）, seRf-repairing group （group C, n= 12, further divided into 7,14,21 d groups） and massage group （group D, n= 12,further divided into 7,14,21 d groups）. In group A,no treatment was made and rabbits were taken as normal controls;in groups B, C, D,rabbit models of quadriceps femoris injury were prepared by self-made beater;in group C,no massage therapy was given and rabbits were taken as natural recovery controls;in group D, massage therapy was given at the 5th after model was built. At 7,14,21 d after injury,d different animal quadriceps samples were taken in groups C and D respectively. [mmunohistochemical method was used to detect expression of bFGF protein. HE staining was employed to inspect pathological changes. Ultrasound imaging was employed to inspect the tissue necrosis situation at 7,14 d after model build. Results ：After massage intervention,expression of bFGF was higher in group D than in group C at 7 d（P=0.016）,higher in group C than in group D at 14,21 d（P=0.000,P=0.020）. Ultrasound of mi- cro-bubble angiography and HE staining showed that injury was lighter,blood supply was richer,necrotic area was significantly de- creased and vascular restored faster in group D compared with those in group C. Conclusions：Massage can effectively prevent muscleatrophy,degeneration and necrosis,provide a rich blood supply for the damage regions and promote the angiogenesis. Mech- anism of beneficial effect of massage on injury repair and neovascularization may be related with bFGF.