RhaFGF联合rhEGF促进兔耳全层皮肤缺损创面愈合的实验研究

Experimental study of rhaFGF combined with rhEGF in promoting wound healing of rabbits' ears with full-thickness skin injury

目的:观察重组人酸性成纤维细胞生长因子(recombint human acidic fibroblast growth factor,rhaFGF)联合重组人表皮细胞因子(recombined human epidermal growth factor,rhEGF)对创面愈合的影响。方法:建立兔耳全层皮肤缺损创面模型,随机分为A组(rhaFGF 60 U/cm2+rhEGF 20 U/cm2)、B组(rhaFGF 60 U/cm2)、C组(重组牛碱性成纤维细胞生长因子60 U/cm2)和D组(等量生理盐水),共4组。采用透明薄膜描记法记录建模后不同时间点各组创面愈合面积,计算创面愈合率,并记录创面愈合时间。在第3、7、14天取创面组织,作HE染色,观察不同时相点创面愈合情况。结果:A组创面愈合时间为(13.33±0.12)d,与其他组比较差异具有统计学意义(P<0.05)。A组创面愈合率与其他组比较,差异具有统计学意义。造模后第3、7天,HE染色示A和C组创面成纤维细胞、毛细血管生成较B、D组丰富。第14天,A组创面基本愈合,成纤维细胞、毛细血管较其他组少。结论:rhaFGF联合rhEGF对兔耳创面愈合有促进作用,可促进创面肉芽组织生长、毛细血管与上皮形成。在创面愈合后期,毛细血管及成纤维细胞生成数目少,有可能减少瘢痕形成。

Objective ：To study the effects of recombinant human acidic fibroblast growth factor（rhaFGF） and recombinant human epi- dermal growth factor（rhEGF） on wound healing of rabbits＇ ears with full-thickness skin injury. Methods：Models of rabbits＇ ears with full-thickness skin injury were made and randomly divided into four groups：group A（rhaFGF 60 U/cm2＋rhEGF 20 U/cm2）, group B （rhaFGF 60 U/em2） ,group C （recombinant bovine basic fibroblast growth factor 60 U/cm2）and group D（equivalent normal saline）. The reagents in the brackets were given every two days. Wound healing rate and time were respectively recorded at different time points through hyaline membrane tracing. Wound tissues were obtained on the 3rd, 7th and 14th d to evaluate wound healing state through HE staining. Results：Wound healing time was statistically different between group A （（ 13.33 ± 0.12） d） and other groups （P〈0.05）. Wound healing rate was also statistically different between group A and other groups. HE staining showed that on the 3rd and 7th d after modeling, growth of fibroblasts and capillary was more vibrate in group A and C than that in group B and D. On the 14th d after modeling,wound was almost healed in group A and fibroblasts and capillary of group A were relatively rare compared with those in other groups. Conclusions：rhaFGF combined with hEGF can promote wound healing in rabbits＇ ears. Combination of the two drugs can stimulate the growth of granulation tissue, capillary formation and re-epithelialization. In the later stage of wound healing, capillary and fibroblasts grow slower,which may reduce the possibility of scar formation.