P300/CBP相关因子在肝细胞癌中的表达及临床意义

Expression of P300/CBP-associated factor in hepatocellular carcinoma and its clinicopathological significance

目的:检测P300/CBP相关因子(P300/CBP-associated factor,PCAF)在肝细胞癌(hepatocellular carcinoma,HCC)中的表达情况,分析其与肝癌临床病理特征之间的相关性。方法:收集40例HCC及对应癌旁组织,运用RT-PCR、免疫组化技术检测PCAF在肝癌及对应癌旁组织中的表达情况,研究PCAF蛋白表达与肝癌临床病理特征之间的相关性;应用Western blot技术检测人正常肝细胞LO2和肝癌细胞HepG2和SMMC-7721中PCAF蛋白的表达。结果:PCAF mRNA及蛋白在肝癌组织中的表达低于对应的癌旁组织(P=0.000);PCAF蛋白低表达与肿瘤肝内转移(r s=0.413,P=0.037)、门脉侵犯(r s=0.589,P=0.011)和高TNM分期(r s=0.513,P=0.029)相关;人肝癌细胞株HepG2和SMMC-7721中PCAF蛋白的表达较正常肝细胞株LO2降低(P=0.000)。结论:肝癌细胞系及肝癌组织中PCAF表达下调,且PCAF的表达下调与肝癌的恶性病理特征相关。

Objective ：To investigate the expression of P300/CBP-associated factor（PCAF） and its correlation with the clinicopatholog- ical features in hepatocellular carcinoma（HCC）. Methods：Forty samples of surgically resected HCC and adjacent noncancerous tis- sues were collected. PCAF expression in the samples was detected using RT-PCR and immunohistochemical staining. Correlation be- tween PCAF protein expression and clinicopathological feature was analyzed. PCAF protein expression in normal hepatic cell line （LO2） and HCC cell lines（HepG2 and SMMC-7721 ） was detected. Results：mRNA and protein expression of PCAF in HCC tissues was significantly down-regulated compared with that of adjacent noncancerous tissues（P〈O.OS,respectively）. Clinicopathological analysis suggested that PCAF protein low-expression was associated with tumor intrahepatic metastasis（rs=O.413,P=0.037）,portal vein invasion （rs=0.589, P=O.011 ） and advanced TNM stage （re=0.513, P=0.029）. Expression of PCAF protein in HCC cell lines HepG2 and SMMC-7721 was significandy lower compared with that in hepatic cell line LO2（P〈0.05,respectively）. Conclusions：Expression of PCAF is down-regulated in HCC cell lines and HCC tissues and the low-expression of PCAF is correlated with poor clinieo- pathological characteristics of HCC.