摘要
目的:检测P300/CBP相关因子(P300/CBP-associated factor,PCAF)在肝细胞癌(hepatocellular carcinoma,HCC)中的表达情况,分析其与肝癌临床病理特征之间的相关性。方法:收集40例HCC及对应癌旁组织,运用RT-PCR、免疫组化技术检测PCAF在肝癌及对应癌旁组织中的表达情况,研究PCAF蛋白表达与肝癌临床病理特征之间的相关性;应用Western blot技术检测人正常肝细胞LO2和肝癌细胞HepG2和SMMC-7721中PCAF蛋白的表达。结果:PCAF mRNA及蛋白在肝癌组织中的表达低于对应的癌旁组织(P=0.000);PCAF蛋白低表达与肿瘤肝内转移(r s=0.413,P=0.037)、门脉侵犯(r s=0.589,P=0.011)和高TNM分期(r s=0.513,P=0.029)相关;人肝癌细胞株HepG2和SMMC-7721中PCAF蛋白的表达较正常肝细胞株LO2降低(P=0.000)。结论:肝癌细胞系及肝癌组织中PCAF表达下调,且PCAF的表达下调与肝癌的恶性病理特征相关。
Objective :To investigate the expression of P300/CBP-associated factor(PCAF) and its correlation with the clinicopatholog- ical features in hepatocellular carcinoma(HCC). Methods:Forty samples of surgically resected HCC and adjacent noncancerous tis- sues were collected. PCAF expression in the samples was detected using RT-PCR and immunohistochemical staining. Correlation be- tween PCAF protein expression and clinicopathological feature was analyzed. PCAF protein expression in normal hepatic cell line (LO2) and HCC cell lines(HepG2 and SMMC-7721 ) was detected. Results:mRNA and protein expression of PCAF in HCC tissues was significantly down-regulated compared with that of adjacent noncancerous tissues(P〈O.OS,respectively). Clinicopathological analysis suggested that PCAF protein low-expression was associated with tumor intrahepatic metastasis(rs=O.413,P=0.037),portal vein invasion (rs=0.589, P=O.011 ) and advanced TNM stage (re=0.513, P=0.029). Expression of PCAF protein in HCC cell lines HepG2 and SMMC-7721 was significandy lower compared with that in hepatic cell line LO2(P〈0.05,respectively). Conclusions:Expression of PCAF is down-regulated in HCC cell lines and HCC tissues and the low-expression of PCAF is correlated with poor clinieo- pathological characteristics of HCC.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2013年第11期1348-1351,共4页
Journal of Chongqing Medical University
关键词
P300
CBP相关因子
肝细胞癌
组蛋白乙酰化
临床意义
P300/CBP-associated factor
hepatocellular carcinoma
histone acetylation
clinical significance