内皮脂肪酶基因584C/T多态性及其血清水平与急性心肌梗死的相关性

EL 584C/T gene polymorphism and acute myocardial infarction

目的:探讨内皮脂肪酶(EL)基因584C/T多态性及其血清水平与急性心肌梗死(AMI)的相关性。方法:采用聚合酶链反应-限制性片段长度多态性方法检测162例中国常州地区汉族人群AMI患者和299例健康体检者EL基因584C/T基因型;采用ELISA法检测血清EL水平。结果:AMI组血清EL水平明显高于对照组;中国常州地区汉族人群存在EL 584C/T基因多态性,基因型及等位基因频率分布符合Hardy-Weinberg平衡。AMI组CC、CT、TT基因型频率分别为62.4%、36.4%、1.2%;对照组CC、CT、TT基因型频率分别为56.2%、40.8%、3.0%;两组间基因型频率分布差异无统计学意义(P=0.293)。AMI组C等位基因频率为80.6%;对照组C等位基因频率为76.6%,两组间等位基因频率差异也无统计学意义(P=0.184)。经校正性别、年龄、糖尿病、高血压、吸烟、血脂异常等冠心病危险因素后,结果仍然表明该多态性与AMI无明显相关性。无论是对照组还是AMI组,与T等位基因携带者比较,CC基因型血清EL水平都无显著性差异。结论:EL 584C/T基因多态性与AMI无明显相关性,与血清EL水平也无相关性,EL 584C/T多态性可能不是中国常州地区汉族人群AMI发病的易感因素。

Objective：To explore the endothelial lipase（EL）584C/T gene polymorphism and relationship between EL level and acute myocardial infarction（AMI）.Method：The EL 584C/T genotypes were detected by polymerase chain reaction-restriction fragment length polymorphism analysis.The EL level was detected by enzyme linked immnunosorbent assay.Result：①The level of EL in AMI group was higher than that in controls.②The frequencies of the CC,CT and TT genotypes were respectively 62.4%,36.4%and 1.2%in the AMI group,and 56.2%,40.8%and 3.0%in control group.The C allele frequency was 80.6% and 76.6%in two groups,respectively.There was no significant difference between AMI and control groups in genotypic and allelic frequencies.In logistic regression,the relationship between the EL 584C/T gene polymorphism and AMI remained no significant difference after adjusting for traditional AMI risk factors.③There was no significant difference among different EL584C/T genotypes in the serum EL level.Conclusion：There is no significant relationship between EL584C/T gene polymorphism and AMI in Changzhou Han population.