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基因谱系示踪揭示Tbx18^+祖细胞的多分化潜能

Genetic Fate Mapping Reveals the Multi-differentiation Potential of Tbx18^+ Progenitor Cells
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摘要 为探讨Tbx18+祖细胞在小鼠生长发育过程中的多分化潜能及分化的组织类型,本工作建立了Tbx18:Cre/Rosa26RLac Z谱系示踪小鼠.该示踪小鼠基于Cre-LoxP系统,能够准确及有效地示踪Tbx18+祖细胞的分化命运,通过整体胚胎及组织X-gal染色,检测分析报告基因LacZ在其中的表达情况.结果显示,在Tbx18:Cre/Rosa26RLac Z双杂合小鼠胚胎发育早期,报告基因LacZ主要在脊柱、四肢及心外膜表达;而在胚胎发育晚期则分别表达于皮肤、毛囊、肾脏、输尿管、膀胱、睾丸、输精管、椎间盘、肋软骨、心耳、心肌、冠状动脉.结果阐明,Tbx18+祖细胞在小鼠生长发育过程中具有强大的多器官及组织分化潜能,包括分化形成表皮系统,泌尿生殖系统,骨骼系统,心血管系统,并在其生长发育中发挥重要作用. To study the multi-differentiation potential and the differentiated tissue types of Tbxl8 ^＋ progenitor cells in the mouse growth and development, Tbxl8： Cre/Rosa26R^Lacz genetic fate-mapping mouse was established. The fate of Tbxl8 ^＋ progenitor cells was traced exactly and effectively based on Cre-LoxP system. LacZ was detected in the whole mouse embryo and tissue followed by X-gal staining. The results revealed that in the model of Tbx18：Cre/Rosa26R^Lacz double-heterozygous mouse, LacZ was expressed in vertebral column, limbs and epicardium in the early gestation stage of the mouse embryo. However, in the late gestation stage, LacZ was detected in cutaneous covering, folliculus pili, kidney, ureter, bladder, testicle, deferent duct, disci intervertebrales, rib cartilage, atrial appendage, myocardium and coronary artery, respectively. The results elucidated that Tbxl8 ^＋ progenitor cells had a great potential in differentiation into multiple organs and tissues, including epidermal, genitourinary, skeletal and cardiovascular system, and played an essential role in the mouse growth and development.
作者 查承沁 杜建霖 翁敏杰 高凌志 汪浩 佘强
机构地区 重庆医科大学附属第二医院心血管内科 北仑区人民医院心血管内科
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2013年第11期1035-1040,共6页 Chinese Journal of Biochemistry and Molecular Biology
基金 国家自然科学基金(No.30971213 No.81270211) 国家自然科学青年基金(No.81100088)~~
关键词 Tbx18 祖细胞 谱系示踪 多分化潜能 T-box transcription factor 18 progenitor cells genetic fate-mapping multi-differentiationpotential
分类号 R363.35 [医药卫生—病理学] R394.3 [医药卫生—医学遗传学]
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参考文献18

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中国生物化学与分子生物学报
2013年 第11期

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