老年黄斑变性外周血中视网膜抗体的研究

Antiretinal antibodies in serum of patients with age related macular degeneration

目的探索老年黄斑变性(AMD)的免疫发病机理。方法运用SDS PAGE电泳、免疫印迹技术检测老年黄斑变性患者外周血中视网膜抗体;运用免疫组化法进行组织定位。结果老年黄斑变性患者外周血中存在显著高于正常对照组的视网膜抗体,其相应抗原相对分子质量为27000～35000,43000～50000,56000～67000,80000～90000,抗原定位在视网膜光感受器外节和神经元的胞核。结论自身免疫反应是老年黄斑变性发生、发展的一个重要因素。

ObjectiveTo evaluate the hypothesis that antiretinal autoimmunity is involved in the development of age related macular degeneration (AMD).MethodsSera from 28 patients with AMD and human retina of 13 control subjects were tested on Western blots for antiretinal antibodies;Serum antibodies from 4 patients with AMD that had shown immunoreactivity with the human retinal homogenate were tested on W estern blots of photoreceptor outer segment homogenate from bovine retina.Froze n sections of human retina were incubated with sera from four of the patients wh ose antibodies had exhibited a strong immunoreactivity to retinal homogenate.ResultsSerum antibodies from patients with AMD were recognized in the proteins of 27000～35000,43000～50000,56000～67 000,80000～90000.Most of positive bands locat ed at 27000～35000,43000～50000.Serum a ntibodies from four patients with AMD th at had shown immunoreactivity with the h uman retinal homogenate also reacted wit h a protein of 56000～67000 on Western blots of photoreceptor outer segment hom ogenate from bovine retina.In AMD cases , labelling was confined to neuropl asm and neuronuclei.ConclusionThe res ults indicate that immunologic factors plays an important role in the pathogen esis of AMD.