摘要
目的建立兔视网膜色素上皮(RPE)细胞的体外标记方法。方法兔RPE细胞经传代培养并鉴定后,掺入溴脱氧嘧啶(BrdU)对细胞进行体外标记,通过免疫组织化学方法来显示标记的细胞。结果BrdU标记后,所有的细胞核呈阳性染色。结论提供一种简便易行的RPE细胞标记方法,提示BrdU掺入法适用于所有培养细胞的体外标记。
ObjectiveTo esta blish a labelling method for rabbit RPE cells in vitro.MethodsRabbit RPE cells were cultured and passaged in vitr o. RPE cells were labelled by BrdU incorporation,identified as RP by imm unocytochemistry methods.ResultsAll the nuclei of the RPE cells showed positive labelling with BrdU.ConclusionThis s tudy provides an easy way for labelling RPE cells,and suggests that cultu red cells could be labelled by BrdU inco rporation.
出处
《眼科研究》
CSCD
2000年第5期421-422,共2页
Chinese Ophthalmic Research
