无籽罗汉果组培无菌系的构建

The Construction of the Aseptic Strain of the Tissue Culture of Seedless Siraitia grosvenorii

研究无籽罗汉果组培快繁技术要点,为无籽罗汉果再生体系建立和工厂化生产提供技术支持。以幼嫩茎段为试验材料,探讨不同灭菌时间,不同激素组合以及浓度对启动培养、增殖培养、生根培养等的影响。无籽罗汉果带芽茎段最佳消毒方法为1%HgCl_2溶液消毒8 min。最适宜的无籽罗汉果茎段启动培养基为MS+1.0 mg/L 6-BA+1.5 mg/L KT,无籽罗汉果继代增殖培养基为MS+1.0 mg/L 6-BA+0.5 mg/L IBA,增殖系数可达6.8;较适合生根的培养基为1/2MS+1.0 mg/L IBA+0.5 mg/L NAA,新稍平均生根数达7.4条,生根率可达95%。可为无籽罗汉果大规模组织培养和快速繁殖提供理论基础,并为组织培养高效体系的建立及外源基因导入提供参考。

This research explored the tissue culture and rapid propagation of seedless Siraitia grosvenorii in order to provide technical supports on seedless Siraitia grosvenorii regenerations system establishment and factory production. The young stem of seedless Siraitia grosvenorii was used as explants to investigate the effects of different sterilization time, different hormone combinations and concentrations on the initiating, proliferation, as well as rooting cluster buds were studied. The results showed that the best sterilization method for the stem segments was agitating in 0.1% HgCl2 for 8 min. The optimum medium for initiation culture of stem segments was MS＋1.0 mg/L 6-BA＋1.5 mg/L KT. The suitable multiplication medium was MS＋1.0 mg/L 6-BA＋0.5 mg/L IBA, with the bud proliferation coefficient of 6.8. The suitable rooting medium was 1/2MS＋1.0 mg/L IBA＋0.5 mg/L NAA, in which the average root number was 7.4, the rooting rate of multiplied buds was up to 95%. It could provide theoretical basis for the large-scale production of the tissue culture and rapid propagation of seedless Siraitia grosvenorii, and the experiment provided references for the establishment of efficient tissue culture regeneration system and the exogenous gene transduction.