外源性孕酮对牛卵母细胞体外核成熟的影响

The Effect of Exogenous Progesterone on in vitro Nuclear Maturation of Bovine Oocytes

为了研究外源性孕酮对牛卵母细胞体外核成熟的作用,通过向成熟培养液中添加10μg/mL FSH＋10μg/mL LH（对照组）和0、10、100、1000 ng/mL的外源性孕酮,对牛卵丘卵母细胞复合体进行体外成熟培养,在培养8 h后用醋酸地衣红染色观察各自的生发泡破裂率,然后在成熟22 h统计第一极体率,体外受精后7~8天统计囊胚率。结果表明：对照组卵母细胞的生发泡破裂率（75.0%）和极体率（79.6%）均明显高于添加不同浓度孕酮的各处理组（P〈0.05）,而囊胚率各组之间均无显著差异（P〉0.05）。因此,外源性孕酮不能促进牛卵母细胞的核成熟,单独添加外源性孕酮不能起到与内源性孕酮相同的作用。

In this experiment,it was aimed to investigate the effect of exogenous progesterone on in vitro nuclear maturation of bovine oocytes through adding with 10 μg/mL FSH ＋ 10 μg/mL LH（control）,0,10,100,1000 ng/mL exogenous progesterone respectively into the in vitro maturation medium for bovine cumulus-oocyte complexes（COCs）.The germinal vesicle breakdown（GVBD） rate of each group after 8 h using acetic acid-orcein staining,and counted first polarbody（PbI） rate after 22 h were all analyzed.Finally,the blastocyst rate after 7-8 days of in vitro fertilization was also counted.The results showed that,GVBD（75.0%） and PbI（79.6%） rate of the control group were significantly higher than the other ones adding with exogenous progesterone（P＆lt;0.05）,and blastocyst rate of each group didn’ t show significant variation（P＆gt;0.05）.Therefore,it could be concluded that the exogenous progesterone could not play a role for improving the nuclear maturation of bovine oocytes,it also meant that exogenous progesterone alone could not take the same effect as endogenous progesterone in such kind of processes.