扶正活血清热方对急性放射性肠炎血清中CAT和MDA的影响

Effects of Fuzhenghuoxueqingrefang on CAT and MDA in the serum of acute radiation enteritis in rats

目的探讨扶正活血清热方治疗大鼠急性放射性肠炎(ARE)的作用机制。方法将32只受试大鼠随机分为4组(8只/组),即空白对照组、模型组、地塞米松组、扶正活血清热方组。模型组、地塞米松组和扶正活血清热方组大鼠于成功造模后第2天开始分别用生理盐水、地塞米松和扶正活血清热方灌肠干预5 d(5毫升/只),于照射后第7天,麻醉后大鼠经采血测定其中过氧化氢酶(CAT)的活性和丙二醛(MDA)的含量。结果空白对照组、地塞米松组和扶正活血清热方组血清中CAT活性明显高于模型组(F=23.79,P<0.01)。空白对照组、地塞米松组和扶正活血清热方组血清中MDA含量均明显低于模型组(F=36.41,P<0.01)。结论扶正活血清热方可能通过下调受损组织中脂质过氧化反应程度来治疗放射性肠炎。

Objective To investigate the treating mechanism of Fuzhenghuoxueqingrefang on acute radiation enteritis（ARE）in rats. Methods Thirty two Wistar male rats were randomly divided into 4 groups（8 rats each group）, which were composed of blank control, model, dexamethasone, and Fuzhenghuoxueqingrefang groups. The physiological saline, dexamethasone and Fuzhenghuoxueqingrefang were administrated separately at the first day after abdominal irradiation by daily rectal clysma for 5 days. All the rats were anesthetized and the serum activity of peroxidase （CAT） and contents of malonic aldehyde（MDA） were detected at the seventh day after abdominal irradiation separately. Results The activity of serum CAT in the blank control, dexamethasone and Fuzhenghuoxueqingrefang groups were obviously higher than that of the model group（F=23.79, P〈0.01）. The content of serum MDA in the blank control, dexamethasone and Fuzhenghuoxueqingrefang groups were significantly lower than that of the model groups（F=36.41, P〈0.01）. Conclusion Fuzhenghuoxueqingrefang could treat ARE in rats by down-regulating the lipid peroxidation in the damaged tissue.