室温对预溶稀释血糖化血红蛋白的影响结果分析

Effects of room temperature on the solubility of diluted blood glycosylated hemoglobin

目的：探讨室温对预溶稀释血样本糖化血红蛋白（HbA1c）结果的影响.方法：分别采用高效液相色普法（HPLC）即刻检测全血和胶乳凝集反应法检测预溶稀释血即刻（5min）、放置室温（温度20℃～25℃,湿度60％-70％）1h、2h、4h、8h及24h时间段内的HbA1c含量并进行比较分析.结果：胶乳凝集反应法与HPLC法检测HbA1c无统计差异（P＜0.05）,两者有很好的相关性,R2=0.9924；预溶稀释血在室温放置各个时间段无统计差异（P＜0.05）.结论：胶乳凝集反应法是测定HbA1c一种操作简单、自动化、重复性好的方法,且预溶稀释血在室温放置24内仍很稳定.

Objective： To investigate the room temperature on the solution diluted blood sample of glycosylated hemoglobin （HbA1 c） results. Methods： HPLC （HPLC） immediate detection of the blood and latex agglutination method detection of solution di- luted blood immediately （5 min）, placed at room temperature （ the temperature of 20℃～25 ℃, humidity 60% - 70% ） 1 h, 2h, 4h, 8h and 24h period of the HbAlc content and comparative analysis. Results： no statistical difference of latex agglutination method and HPLC method to detect HbAlc （P 〈 0. 05）, there is a correlation between the two, good R2 =0. 9924; the solution diluted blood at room temperature for various time periods of no statistical difference （ P 〈 0. 05 ） . Conclusion ： latex agglutination method is a simple method for the determination of HbA1 c, automation, good repeatability, and the solution diluted blood at room temperature for 24 is still very stable.